第25回生命分子化学セミナー
『Distinct p53 Genomic Binding Patterns in Normal and Cancer-derived Human Cells』
| 講演者 | Dr. Carl W. Anderson Brookhaven National Laboratory, USA |
| 日時 | 平成２３年１０月２５日（火） 15:00 - |
| 場所 | 北海道大学理学部 2号館402室 |
| 要旨 | The p53 tumor suppressor is a tetrameric transcription factor. Genome-wide analysis of its binding sites in normal human IMR-90 fibroblasts revealed 743 high-confidence ChIP-seq peaks representing putative p53 binding sites. More than 40% were located within 2 kb of a transcription start site (TSS), a distribution similar to that documented for individually studied, functional p53 response elements. Nearly half of the peaks reside in CpG islands, in marked contrast to sites reported in cancer-derived cells. The distinct genomic features of the IMR-90 binding sites do not reflect a distinct preference for specific sequences since the de novo developed p53 motif based on our study is similar to those reported by genome-wide studies of cancer-derived cells. More likely, the different chromatin landscape in normal compared to cancer-derived cells influences p53 binding via modulating the availability of sites through epigenetic mechanisms. We compared the IMR-90 ChIP-seq peaks to the recently published IMR-90 methylome and demonstrate that they are enriched at hypomethylated DNA. A hypothesis for the differences in p53 binding between normal human cells and cancer-derived cell lines will be presented. |
| 主催 | グローバルCOE物質科学イノベーション講演会 |
| 共催 | 日本生化学会北海道支部 生命分子化学セミナー |
| 連絡先 | 坂口和靖 北海道大学大学院理学研究院化学部門 札幌市北区北10条西8丁目 TEL: 011-706-2698, FAX: 011-706-4683 e-mail: kazuyasu@sci.hokudai.ac.jp |