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2019年(平成31年)
Kamada R., Kimura N., Yoshimura F., Tanino K., Sakaguchi K., Inhibition of Lipid Droplet Formation by Ser/Thr Protein Phosphatase PPM1D Inhibitor, SL-176.  PLOS ONE, 10.1371/journal.pone.0212682

2018年(平成30年)
Ogasawara S., Chuman Y., Michiba T., Kamada R., Imagawa T. and Sakaguchi K., Inhibition of protein phosphatase PPM1D enhances retinoic acid-induced differentiation in human embryonic carcinoma cell line. J. Biochem., DOI 10.1093/jb/mvy119

Kamada R., Yang W., Zhang Y., Patel M.C., Yang Y., Ouda R., Dey A., Wakabayashi Y., Sakaguchi K., Fujita T., Tamura T., Zhu J., Ozato K., Interferon stimulation creates chromatin marks and establishes transcriptional memory Proc. Natl. Acad. Sci. USA, 10.1073/pnas.1720930115

2017年(平成29年)
Toguchi Y., Kamada R., Kanno M., Imagawa T., Sakaguchi K., Quantitative Single Cell Analysis for Transcriptional Activity of p53 Hetero-tetramers between Wild-type Protein and Oligomerization Domain Chemistry Letters, 10.1246/cl.170980.

Wolf G., Rebollo R., Karimi M., Ewing A., Kamada R., Wu W., Wu B., Bachu M., Ozato K., Faulkner G., Mager D., Lorincz M., Macfarlan T. On the role of H3.3 in retroviral silencing. Nature, 548:E1, 2017.

Kamada R., Kudoh F., Yoshimura F., Tanino K., Sakaguchi K. Inhibition of Ser/Thr phosphatase PPM1D induces neutrophil differentiation in HL-60 cells.  J. Biochem., 162: 303-308, 2017.(Free Access)

Sakaguchi T., Janairo, J. I. B., Lussier-Price M. Wada J., Omichinski J.G., Sakaguchi K. Oligomerization enhances the binding affinity of a silver biomineralization peptide and catalyzes nanostructure formation. Sci. Rep., 7: 1400, 2017.(Free Access)

Kamada R., Nakagawa N., Oyama T., Sakaguchi K. Heterochiral Jun and Fos bZIP peptides form a coiled-coil heterodimer that is competent for DNA binding. J. Pept. Sci., 23: 644-649, 2017.

2016年(平成28年)
Kozakai Y., Kamada R., Furuta J., Kiyota Y., Chuman Y., Sakaguchi K. PPM1D controls nucleolar formation by up-regulating phosphorylation of nucleophosmin.  Sci. Rep., 6: 33272, 2016.(Free Access)

Sakai H., Watanabe K., Kudoh F., Kamada R., Chuman Y., Sakaguchi K. Patterning nanowires through the templated growth of multiple modified amyloid peptides.  Sci. Rep., 6: 31993, 2016.(Free Access)

Kamada R., Tano F., Kudoh F., Kimura N., Chuman Y., Osawa A., Namba K., Tanino K., Sakaguchi K. Effective Cellular Morphology Analysis for Differentiation Processes by a Fluorescent 1,3a,6a-Triazapentalene Derivative Probe in Live Cells.  PLOS ONE, 11: e0160625, 2016.(Free access)

Wada J., Miyazaki H., Kamada R., Sakaguchi K. Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro. Chem. Lett., 45: 185-187, 2016.

Kamada, R., Toguchi, Y., Nomura, T., Imagawa, T., Sakaguchi, K. Tetramer Formation of Tumor Suppressor Protein p53: Structure, Function, and Applications. Biopolymers, 106: 598-612, 2016.

2015年(平成27年)
Ogasawara, S., Kiyota, Y., Chuman, Y., Kowata, A., Yoshimura, F., Tanino, K., Kamada, R., and Sakaguchi, K. Novel Inhibitors Targeting PPM1D Phosphatase Potently Suppress Cancer Cell Proliferation. Bioorg. Med. Chem., 23: 6246-6249, 2015.

Cappadocia, L., Mascle, X.H., Bourdeau, V., Tremblay-Belzile, S., Chaker-Margot, M., Lussier-Price, M., Wada, J., Sakaguchi, K., Aubry M., Ferbeyre G. and Omichinski J.G. Structural and Functional Characterization of the Phosphorylation-dependent Interaction between PML and SUMO1. Structure, 23: 126-138, 2015.

Sakaguchi, T., Mine, K., Kudoh, F., Kamada, R., and Sakaguchi, K. Antiproliferative Activity of Silver Nanoplates on Human Promyelocytic Leukemia Cell Lines. Chem. Lett., 44: 327-329, 2015.

2014年(平成26年)
Kozakai, Y., Kamada, R., Kiyota. Y., Yoshimura, F., Tanino, K. and Sakaguchi, K. Inhibition of C-terminal Truncated PPM1D Enhances the Effect of Doxorubicin on Cell Viability in Human Colorectal Carcinoma Cell Line. Bioorg. Med. Chem. Lett., 24: 5593-5596, 2014.

Janairo, J. I. B., Sakaguchi, T., Hara, K., Fukuoka, A. and Sakaguchi, K. Effects of Biomineralization Peptide Topology on the Structure and Catalytic Activity of Pd Nanomaterials. Chem. Commun., 50: 9259-9262, 2014.

Janairo, J. I. B. and Sakaguchi, K. Effects of Buffer on the Structure and Catalytic Activity of Palladium Nanomaterials Formed by Biomineralization. Chem. Lett., 43: 1315-1317, 2014.

2013年(平成25年)
Chuman, Y., Ueyama, M., Sano, S., Wu, F., Kiyota, Y., Higashi, T., Osada, S., and Sakaguchi, K. Effects of E/Z Configuration of Fluoroalkene-containing HDAC Inhibitors on Selectivity for HDAC Isoforms. Chem. Lett., 42: 833-835, 2013.

Sakai, H., Watanabe, K., Asanomi, Y., Kobayashi, Y., Chuman, Y., Shi, L., Masuda, T., Wyttenbach, T., Bowers, MT., Uosaki, K., and Sakaguchi, K. Formation of Functionalized Nanowires by Control of Self-Assembly Using Multiple Modified Amyloid Peptides. Adv. Funct. Mater., 23: 4881-4887, 2013.

2012年(平成24年)
Wada, J., Kamada, R., Chuman, Y., Imagawa,T., and Sakaguchi, K. Inhibition of Tumor Suppressor Protein p53-dependent Transcription by a Tetramerization Domain Peptide via Hetero-oligomerization. Bioorg. Med. Chem. Lett., 22: 2780-2783, 2012.

Yagi, H., Chuman, Y., Kozakai, Y., Imagawa, T., Takahashi, Y., Yoshimura, F., Tanino, K., and Sakaguchi, K. A Small Molecule Inhibitor of p53-inducible Protein Phosphatase PPM1D. Bioorg. Med. Chem. Lett., 22: 729-732, 2012.

2011年(平成23年)
Chuman, Y., Iizuka, K., Honda, T., Onoue, H., Shimohigashi, Y., and Sakaguchi, K. Phosphatase assay for multi-phosphorylated substrates using phosphatase specific-motif anitibody. J. Biochem., 150: 319-325, 2011.

Han, Y., Noguchi, H., Sakaguchi, K., and Uosaki K. Formation process and solvent-dependent structure of a polyproline self-assembled monolayer on a gold surface. Langmuir, 27: 11951-11957, 2011.

Nomura, T., Kamada, R., Ito, I., Sakamoto, K., Chuman, Y., Ishimori, K., Shimohigashi, Y., and Sakaguchi, K. Probing Phenylalanine Environments in Oligomeric Structures Phenylalanine with Pentafluorophenylalanine and Cyclohexylalanine. Biopolymers, 95: 410-419, 2011.

Kamada, R., Nomura, T., Anderson Carl W., and Sakaguchi, K. Cancer-associated p53 Tetramerization Domain Mutants Quantitative Analysis Reveals a Low Threshold for Tumor Suppressor Inactivation. J. Biol. Chem., 286: 252-258, 2011.

Martin-Manso, G., Calzada, M.J., Chuman, Y., Sipes, J.M., Xavier, C.P., Wolf, V., Kuznetsova, S.A., Rubin, J.S., and Roberts, D.D. sFRP-1 binds via its netrin-related motif to the N-module of thrombospondin-1 and blocks thrombospondin-1 stimulation of MDA-MB-231 breast carcinoma cell adhesion adn migration. Arch. Biochem. Biophys., 509:147-156, 2011.

2010年(平成22年)
Nomme, J., Renodon-Corniere, A., Asanomi, Y., Sakaguchi, K., Stasiak, AZ., Stasiak, A., Norden, B., Tran, V., and Takahashi, M. Design of potent inhibitors of human RAD51 recombinase based on BRC motifs of BRCA2 protein: modelling and experimental validation of a chimera peptide. J. Med. Chem., 53: 5782-5791, 2010.

Kamada, R., Yoshino, W., Nomura, T., Chuman, Y., Imagawa, T., Suzuki, T., and Sakaguchi, K. Enhancement of transcriptional activity of mutant p53 tumor suppressor protein through stabilization of tetramer formation by calix[6]arene derivatives. Bioorg. Med. Chem. Lett., 20: 4412-4415, 2010.

Asanomi, Y., Kobayashi, Y., Sakai, H., Masuda, T., Chen, X., Chuman, Y., Uosaki, K., and Sakaguchi, K. Drastic Effects on Fibril Formation of Amyloid-beta Peptides by the Addion of Amino Acid Residue Units to the Termini. Protein Pept. Lett., 17: 458-463, 2010.

Osada, S., Sano, S., Ueyama, M., Chuman, Y., Kodama, H., and Sakaguchi, K. Fluoroalkene modification of mercaptoasetamide-based histone deacetylase inhibitors. Bioorg. Med. Chem., 15 :605-611, 2010.

Kajihara, Y., Murakami, M., Imagawa, T., Otsuguru, K., Ito, S., and Ohta, T. Histamine Potentiates Acid-Induced Responses Mediating Transient Receptor Potential V1 in Mouse Primary Sensory Neurons. Neuroscience, 166, 292-304 (2010).

2009年(平成21年)
Muscolini, M., Montagni, E., Caristi, S., Nomura, T., Kamada, R., Di Agostino, S., Corazzaari, M., Piacentini, M., Blandino, G., Costanzo, A., Sakaguchi, K., and Tuosto, L. Characterization of a new cancer-associated mutant of p53 with a missense mutation (K351N) in the tetramerization domaiin. Cell Cycle, 8: 3396-405, 2009.

Kamada, R., Terai, T., Nomura, T., Chuman, Y., Imagawa, T., and Sakaguchi, K. Effects of tumor-associated mutations in the p53 tetramerization domein on oligomerization state and transcriptional activity. Adv. Exp. Med. Biol., 611: 567-8, 2009.

Nomura, T., Kamada, R., Ito, I., Chuman, Y., Shimohigashi, Y., Sakaguchi, K. Oxidation of methionine residue at hydrophobic core destabilizes p53 tetrameric structure. Biopolymers, 91:78-84, 2009.

Imagawa, T., Terai, T., Yamada, Y., Kamada, R., Sakaguchi,K. Evaluation of Transcriptional Activity of p53 in Individual Living Mammalian Cells. Anal. Biochem., 387:249-56, 2009.

Chuman, Y., Kurihashi, W., Mizukami, Y., Nashimoto, T., Yagi, H., Sakaguchi, K. PPM1D430, a Novel Alternative Splicing Variant of the Human PPM1D, can Dephosphorylate p53 and Exhibits Specific Tissue Expression. J. Biochem., 145:1-12, 2009.

2008年(平成20年)
Chuman, Y., Yagi, H., Fukuda, T., Nomura, T., Matsukizono, M., Shimohigashi, Y., Sakaguchi, K. Characterization of the active site and a unique uncompetitive inhibitor of the PPM1-type protein phosphatase PPM1D. Protein Pept. Lett., 15:938-948, 2008.

Polley, S., Guha, S., Kar, S., Roy, N.S., Sakaguchi, K., Chuman, Y.,Swaminathan, V., Kundu, T., Roy, S. Differential Recognition of Phosphorylated Transactivation Domains of p53 by Different p300 Domains. J. Mol. Biol., 376:8-12 2008.

2007年(平成19年)
Hunt, D.F., Henderson, R.A., Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, A.L., Appella, E., Engelhard, V.H. Pillars article: Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry :Science, 255: 1261-1263, 1992. J. Immunol., 179:2669-2671, 2007.

Honda,T., Shirasu, N., Isozaki, K., Kawano, M., Shigehiro, D., Chuman, Y., Fujita, T., Nose, T., and Shimohigashi, Y. Differential Recognition of Phosphorylated Transactivation Domains of p53 by Different p300 Domains. Bioorg. med. Chem, 15:3883-3888, 2007.

2006年(平成18年)
Higashimoto, Y., Asanomi, Y., Takakusagi, S., Lewis, M.S., Uosaki, K., Durell, S.R., Anderson, C.W., Appella, E., Sakaguchi, K. Unfolding, aggregation, and amyloid formation by the tetramerization domain from mutant p53 associated with lung cancer. Biochemistry, 45, 1608-1619, 2006.

Tanoue, K., Kaya, S., Hayashi, Y., Abe, K., Imagawa, T.,. Taniguchi, K., Sakaguchi, K. New Evidence for ATP Binding Induced Catalytic Subunit Interactions in Pig Kidney Na/K-ATPase. J. Biochem., 140, 1646-1656, 2006.

Honda, T., Matsushima, A., Sumida, K., Chuman, Y., Sakaguchi, K., Onoue, H., Meinertzhagen, I.A., Shimohigashi, Y., Shimohigashi. M. Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies. J. Comp. Neurol., 499, 404-421, 2006.

2005年(平成17年)
Imagawa, T., Yamamoto, T., Kaya, S., Sakaguchi, K., and Taniguchi, K. Thr-774 (Transmembrane Segment M5), Val-920 (M8), and Glu-954 (M9) Are Involved in Na+ Transport, and Gln-923 (M8) Is Essential for Na,K-ATPase Activity. J. Biol. Chem., 280: 18736-18744, 2005.

Abe, K., Kaya, S., Taniguchi, K., Hayashi, Y., Imagawa, T., Kikumoto, M., Oiwa, K. and Sakaguchi, K. Evidence for a Relationship between Activity and the Tetraprotomeric Assembly of Solubilized Pig Gastric H/K-ATPase. J. Biochem., 138: 293-301, 2005.

2004年(平成16年)以前
Kuwata, K.,Gongora, C., Kanno, Y., Sakaguchi, K., Tamura, T., T, Kanno, T., Basrur, V., Martinez, R.,Appella, E., Golub, T. and Ozato, K.: Gamma Interferon g triggers the interaction between ICSBP (IRF-8) and TEL recruiting the histone deacetylase HDAC3 to the interferon responsive element. Mol. Cell. Biol. 22:7439-7448, 2002.

Kawano, M., Okada, K., Honnda, T., Nose, T., Sakaguchi, K., Costa, T. and Shimohigashi, Y.: Structural Requirements of Nociceptin Antagonist Ac-RYYRIK-NH2 for Receptor Binding. J. Peptide Sci. 8:561-569, 2002.

Kar, S., Sakaguchi, K., Shimohigashi, Y., Samaddar, S., Banerjee, R., Basu, G., Swaminathan, V., Kundu, T. K. and Roy, S.: Effect of Phosphorylation on the Structure and Fold of Transactivation domain of p53. J. Biol. Chem. 277: 15579-15585, 2002.

Higashimoto, Y., Saito, S., Tong, X.-H., Hong, A., Sakaguchi, K., Appella, E., and Anderson, C.W.: Human p53 is phosphorylated on Serine 6 and 9 in response to DNA Damage-Inducing Agents. J. Biol. Chem. 275: 23199-23203, 2000.

Sakaguchi, K., Saito, S., Higashimoto, Y., Roy, S. Anderson, C.W., Appella, E.:Damage-mediated phosphorylation of human p53 threonine 18 through a cascade mediated by a casein 1-like kinase. Effect on Mdm2 binding. J. Biol. Chem. 275: 9278-9283, 2000.

Bergman, A.C., Benjamin, T., Alaiya, A., Waltham, M., Sakaguchi, K., Franzen, B., Linder, S., Bergman, T., Auer, G., Appella, E., Wirth, P.J., and Jornvall, H.: Identification of gel-separated tumor marker proteins by mass spectrometry. Electrophoresis 21 : 679-686, 2000.

Appella E, Arnott D, Sakaguchi, K, and Wirth PJ.: Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis. EXS 88: 1-27, 2000.

Bulavin, D. V., Saito, S., Hollander, M.C., Sakaguchi, K., Anderson, C. W., Appella, E., and Fornace, A. J., Jr.: Phosphorylation of human p53 by p38 kinase coordinates N-terminal phosphorylation and apoptosis in response to UV radiation. EMBO J. 18: 6845-6854, 1999.

Mazur, S. J., Sakaguchi, K., Appella, E., Wang, X. W., Harris, C. C., and Bohr, V. A.: Preferential Binding of Tumor Suppressor p53 to Positively or Negatively Supercoiled DNA Involves the C-Terminal Domain. J. Mol. Biol. 292: 241-249, 1999.

Jimenez, G. S., Bryntesson, F., Torres-Arzayus, M. I., Priestley, A., Beeche, M., Saito, S., Sakaguchi, K., Appella, E., Jeggo, P. A., Taccioli, G. E., Wahl, G. M., and Hubank, M: DNA-dependent protein kinase is not required for the p53-dependent response to DNA damage. Nature 400: 81 - 83, 1999.

Delphin, C., Ronjat, M., Deloulme, J. C., Garin, G., Debussche, L., Higashimoto, Y., Sakaguchi, K., and Baudier, J.: Calcium-dependent interaction of S100B with the C-terminal domain of the tumor suppressor p53. J. Biol. Chem. 274: 10539-10544, 1999.

Chuman, Y, Bergman, A. C, Ueno, T., Saito, S., Sakaguchi, K., Alaiya, A. A., Franzen, B., Bergman, T., Arnott e, D., Auer d, G., Appella, E., Jornvall, H., Linder, S.: Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas. FEBS Lett. 462: 129-134, 1999.

Pike, S.E., Yao, L., Setsuda, J., Jones, K.D., Cherney, B., Appella, E., Sakaguchi, K., Nakhasi H., Atreya C.D., Teruya-Feldstein J., Wirth P., Gupta G., Tosato G.: Calreticulin and Calreticulin Fragments Are Endothelial Cell Inhibitors That Suppress Tumor Growth. Blood 94: 2461-2468, 1999.

Herrera, J. E., Sakaguchi, K., Bergel, M., Trieschmann, L., Nakatani, Y., and Bustin, M. Specific acetylation of chromosomal protein HMG-17 by PCAF alters its interaction with nucleosomes. Mol. Cell. Biol., 19: 3466-3473, 1999.

Bergman, A. C., Alaiya, A. A., Wendler, W., Binetruy, B., Shoshan, M., Sakaguchi, K., Bergman, T., Kronenwett, U., Auer, G., Appella, E., Jornvall, H., and Linder, S.:Protein kinase-dependent overexpression of the nuclear protein pirin in c-JUN and RAS transformed fibroblasts. Cell. Mol. Life Sci., 55: 467-471, 1999.

Sakaguchi, K., Herrera, J. E., Saito, S, Miki, T, Bustin, M., Vassilev, A., Anderson, C. W., and Appella, A.: DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev. 12: 2831-2841, 1998.

Canman, C. E., Lim, D-S., Cimprich, K. A., Katsuyuki, Y. T., Tamai, K., Sakaguchi, K., Appella, E., Kastan, M. B., and Siliciano, J. D.: Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science 281: 1677-1679, 1998.

Pise-Masison, C. A., Radonovich, M., Sakaguchi, K., Appella, E., Brady, J. N.: Phosphorylation of p53: a novel pathway for p53 inactivation in human T-cell lymphotropic virus type 1-transformed cells. J. Virol. 72: 6348-6355, 1998.

Munoz-Barroso, I., Durell, S., Sakaguchi, K., Appella, E., Blumenthal, R.: Dilation of the human immunodeficiency virus-1 envelope glycoprotein fusion pore revealed by the inhibitory action of the a synthetic peptide from gp41. J. Cell Biol. 140: 315-323, 1998.

Kawakami, Y., Robbins, P. F., Wang, X., Tupesis, J. P., Parkhurst, M. R., Kang, X. Q., Sakaguchi, K., Appella, E., and Rosenberg, S. A.: Identification of new melanoma epitopes on melanosomal proteins recognized by tumor infiltrating T lymphocytes restricted by HLA-A1, -A2, and -A3 alleles. J. Immunol. 161: 6985-6992, 1998.

Kim, P. J., Sakaguchi, K., Sakamoto, H., Saxinger, C., Day, R., McPhie, P., Rubin, J. S., and Bottaro, D. P.: Colocalization of heparin and receptor binding sites on keratinocyte Growth factor. Biochemistry 37: 8853-8862, 1998.

Pike, S. E., Yao, L., Jones, K. D., Cherney, B., Appella, E., Sakaguchi, K., Nakhasi, H., Teruya-Feldstein, J., and Wirth, P.: Vasostatin, a calreticulin fragment, inhibits angiogenesis and suppresses tumor growth. J. Exp. Med. 188: 2349-2356, 1998.

Siliciano, J. D., Canman, C. E., Taya, Y., Sakaguchi, K., Appella, E., Kastan, M. B.: DNA damage induces phosphorylation of the amino terminus of p53. Genes Dev. 11: 3471-3481, 1997.

Sakaguchi, K., Sakamoto, H., Lewis, M. S., Anderson, C. W., Erickson, J. W., Appella, E., and Xie, D.: Phosphorylation of serine 392 stabilizes the tetramer formation of tumor suppressor protein p53. Biochemistry 36: 10117-10124, 1997.

Fiscella, M., Zhang, H.-L., Fan, S., Sakaguchi, K., Shen, S., Mercer, W. E., Vande Woude, G. F., O'Connor, P. M., and Appella, E.: Wip1, a novel human protein phosphatase that is induced in response to IR in a p53-dependent manner. Proc. Natl. Acad. Sci. USA 12: 6048-6053, 1997.

Sakaguchi, K., Sakamoto, H., Xie, D., Erickson, J. W., Lewis, M. C., Anderson, C. W., and Appella, E.: Effect of phosphorylation of tetramerization of the tumor suppressor protein p53. J. Protein Chem. 6: 553-556, 1997.

Del Guercio, M-F., Alexander, J., Kubo, R., Arrhenius, T., Maewal, A., Appella, E., Hoffman, S., Jones, T., Valmori, D., Sakaguchi, K., Grey, H. M., Sette, A.: Potent immunogenic short linear peptide constucts composed of B cell epitopes and Pan DR T Helper Epitopes (PADRE) for antibody responses in vivo. Vaccine 15: 441-448, 1997.

Tsai, V., Southwood, S., Sidney, J., Sakaguchi, K., Kawakami, Y., Appella, E., Sette, A., and Celis, E.: Identification of subdominant CTL epitopes of the GP100 melanoma-associated tumor antigen by primary in vitro immunization with peptide-pulsed dendritic cells. J. Immunol. 158: 1796-1802, 1997.

Bergman, A. C., Linder, C., Sakaguchi, K., Sten-Linde, R. M., Alaiya, A. A., Franzen, B., Shoshan, M. C., Bergman, T., Wiman, B., Auer, G., Appella, E,. Jornvall, H., and Linder S.: Increased expression of alpha-enolase in c-jun transformed rat fibroblasts without increased activation of plasminogen. FEBS Lett. 417:17-20, 1997.

Kimura, T., Zhang, J., Sagawa, K., Sakaguchi, K., Appella, E., and Siraganian, R. P.: Syk-independent tyrosine phosphorylation and association of the protein tyrosine phosphatases SHP-1 and SHP-2 with the high affinity IgE receptor. J. Immunol. 59: 4426-4434, 1997.

Miller, M., Lubkowski, J. , Mohana Rao, J. K., Danishefsky, A. T., Omichinski, J. G., Sakaguchi, K., Sakamoto, H., Appella, E., Gronenborn, A. M., and Clore, G. M.: The oligomerization domain of p53: crystal structure of the trigonal form. FEBS Lett. 399: 166-170, 1996.

Sakamoto, H., Kodama, H., Higashimoto, Y., Kondo, M., Lewis, M. S., Anderson, C. W., Appella, E. and Sakaguchi, K.: Chemical synthesis of phosphorylated peptides of the carboxy-terminal domain of human p53 by a segment condensation method. Int. J. Pept. Protein Res. 48: 429-442, 1996.

Di Marzo Veronese, F., Arnott, D., Barnaba, V., Loftus, D. J., Sakaguchi, K., Thompson, C. B., Salemi, S., Mastroianni, C., Sette, A., Shabanowitz, J., Hunt, D. F., and Appella, E.: Autoreactive cytotoxic T lymphocytes in human immunodeficiency virus type-1 infected subjects. J. Exp. Med. 183: 2509-2516, 1996.

Fineschi, B., Sakaguchi, K., Appella, E., and Miller, J.: The proteolytic environment involved in MHC class II-restricted antigen presentation can be modulated by the p41 forms of invariant chain. J. Immunol. 157: 3211-3215, 1996.

D'Oro, U., Sakaguchi, K., Appella, E. and Ashwell, J. D.: Mutational analysis of Lck in CD45-negetive T cells. Mol. Cell. Biol. 16: 4996-5003, 1996.

Sakaguchi, K., Roller, P. P. and Appella, E.: Chemical synthesis and applications of phosphopeptides. In Setlow J. K. (Ed.): Genetic Engineering: Principles and Methods, Vol. 18, New York, Plenum, pp. 249-278, 1996.

Clubb, T. R., Omichinski, J. G., Sakaguchi, K., Appella, E., Gronenborn, M. A. and Clore, G. M.: Backbone dynamics of the oligomerization domain of p53 determined from 15N NMR relaxation measurements. Protein Science 4; 855-862, 1995.

Clore, G. M., Ernst, J., Clubb, R., Omichinski, J. G., Sakaguchi, K., Appella, E. and Gronenborn, A. M.: Refined solution structure of the oligomerization domain of the tumor suppressor p53. Nature Struct. Biol. 2: 321-338, 1995.

Clore, G. M., Omichinski, J. G., Sakaguchi, K., Zambrano, N., Sakamoto, H., Appella, E. and Gronenborn, A. M.: Interhelical angles in the solution structure of the oligomerization domain of the tumor suppressor p53. Science 267: 1515-1516, 1995.

Kang, X., Kawakami, Y., Sakaguchi, K., Wang, R., El-Gamil, M., Yannelli, R. J., Appella, E., Rosenberg, S. A. and Robbins, P. F.: Identification of a tyrosinase epitope recognized by HLA-A24 restricted tumor-infiltrating lymphocytes. J. Immunol. 155: 1343-1348, 1995.

Robbins, P. F., El-Gamil, M., Yong, L., Topalian, S., Rivoltini, L., Sakaguchi, K., Appella, E., Kawakami, Y. and Rosenberg, S. A.: Cloning of a new gene recognized by melanoma-specific HLA-A24 restricted tumor infiltrating lymphocytes. J. Immunol. 154: 5944-5950, 1995.

Grohmann, U., Puccetti, P., Belladonna, M. L.., Fallarino, F., Bianchi, R., Binaglia, L., Sakaguchi, K., Mage, M. G., Appella, E. and Fioretti, M. C.: Multiple point mutations in an endogenous retroviral gene confer high immunogenicity on a drug-treated murine tumor. J. Immunol. 154: 4631-4641, 1995.

Rivoltini, L., Kawakami, Y., Sakaguchi, K., Southwood, S., Sette, A., Robbins, P. F., Marincola, F. M., Salgaller, M. L., Yannelli, J. R., Appella, E. and Rosenberg, S. A.: Induction of tumor reactive CTL from peripheral blood and tumor infiltrating lymphocytes of melanoma patients by in vitro stimulation with an immunodominant peptide of the human melanoma antigen MART-1. J. Immunol. 154: 2257-2265, 1995.

Kawakami, Y., Eliyahu, S., Jennings, C., Sakaguchi, K., Kang, X., Southwood, S., Robbins, P. F., Sette, A., Appella, E. and Rosenberg, S. A.: Recognition of multiple epitopes in the human melanoma antigen gp100 by tumor infiltrating T-lymphocytes associated with in vivo tumor regression. J. Immunol. 154: 3961-3968, 1995.

Loftus, J. D., Kubo, R. T., Sakaguchi, K., Celis, E., Sette, A. and Appella, E.: Analysis of MHC-Specific Peptide Motifs: Applications in Immunotherapy. Adv. Exp. Med. Biol. 383: 201-210, 1995.

Appella, E., Loftus, J. D., Sakaguchi, K., Sette, A. and Celis, E.: Synthetic Antigenic Peptides as a New Strategy for Immunotherapy of Cancer. Biomedical Peptides, Proteins and Nucleic Acids, 1: 177-184, 1995.

Clore, G. M., Omichinski, J. G., Sakaguchi, K., Zambrano, N., Sakamoto, H., Appella, E., and Gronenborn, A. M.: High-resolution solution structure of the oligomerization domain of p53 by multi-dimentional NMR. Science 265: 386-391, 1994.

Sakamoto, H., Lewis, M. S., Kodama, H., Appella, E., and Sakaguchi, K.: Specific sequences from the carboxy-termines of human p53 form anti-parallel tetramers in solution. Proc. Natl. Acad. Sci. USA 91: 8974-8978, 1994.

Wondrak, E. M., Sakaguchi, K., Rice, W. G., Kun, E., Kimmel, A. R., and Louis, J. M.: Removal of zinc is required for processing of the mature nucleocapsid protein of human immunodeficiency virus, type 1, by the viral protease. J. Biol. Chem. 269: 21948-21950, 1994.

Kawakami, Y., Eliyahu, S., Sakaguchi, K., Robbins, P. F., Rivoltini, L., Yannelli, J. R., Appella, E., and Rsenberg, S. A.: Identification of the immunodominat peptides of the MART-1 human melanoma antigen recognized by the majority of HLA-A2 restricted tumor infiltrating lymphocytes. J. Exp. Med. 180: 347-352, 1994.

Kawakami, Y., Eliyahu, S., Delgado, C. H., Robbins, P. F., Sakaguchi, K., Appella, E., Yannelli, J. R., Adema, G. J., Miki, T., and Rosenberg, S. A.: Identification of a melanoma antigen recognized by tumor infiltrating lymphocytes associated with in vivo tumor rejection. Proc. Natl. Acad. Sci. USA 91: 6458-6462, 1994.

Sidney, J., Oseroff, C., Del Guercio, M.-F., Southwood S., Krieger J. I., Ishioka, G. Y., Sakaguchi, K., Appella, E., and Sette A.: Definition of a DQ3.1 specific binding motif. J. Immunol. 152: 4516-4525, 1994.

Franco, A., Appella, E., Kagnoff, M. F., Chowers, Y., Sakaguchi, K., Grey, H. M., Sette, A.: Peripheral T-Cell Response to A-gliadin in Celiac Disease. Differential processing and presentation capacities of Epstein-Barr transformed B cells and fibroblasts. Clinical Immunology and Immunopathology 71: 75-81, 1994.

Kubo, R. T., Sette, A., Grey, H. M., Appella, E., Sakaguchi, K., Zhu, N.-Z., Arnott, D., Sherman, N., Shabanowitz, J., Michel, H., Bodnar, W. M., Davis, T. A., and Hunt, D. F.: Definition of specific peptide motifa for four major HLA-A alleles. J. Immunol. 152: 3913-3924, 1994.

Chen, Y., Sidney, J., Southwood, S., Cox, A. L., Sakaguchi, K., Henderson, R. A., Appella, E., Hunt D. F., Sette, A., and Engelhard, V. H.: Naturally processed peptides longer than 9 amino acid residues bind to the class I MHC molecule HLA-A2.1 with high affinity and in different conformations. J. Immunol. 152: 2874-2881, 1994.

Sakaguchi, K., Kodama, H., Ogino, Y., Costa, T., Nose, T., and Shimohigashi, Y.: Structural essentials of Ser-1 in tethered peptide ligand of human thrombin. Bull. Chem. Soc. Jpn. 67: 1052-1056, 1994.

Burns, C. M., Sakaguchi, K., Appella, E., and Ashwell J. D.: CD45 regulation of COOH-terminal tyrosine phosphorylation and enzyme activity of src family kinases. J. Biol. Chem. 269: 13594-13600, 1994.

Bovolenta, C., Driggers, P. H., Marks, M. S., Medin, J. A., Politis, A. D., Vogel, S. N., Levy, D. E., Sakaguchi, K., Appella, E., Coligan J. E., and Ozato, K.: Molecular interactions between ICSBP and members of the interferon regulatory factor family. Proc. Natl. Acad. Sci. USA 91: 5046-5050, 1994.

Hipps, D. S., Packman, L. C., Sakaguchi, K., Appella, E., and Perham, R. N.: The peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase complex of Bacillus stearothermorphilius:: Preparation and characterization of its binding to the dihydrolipotl dehydrogenase component. Biochem. J. 297: 137-143, 1994.

Ullrich, S. J., Sakaguchi, K., Lees-Miller, S., Anderson, C., Mercer, W. E., and Appella, E.: Phosphorylation of serines 15 and 392 in mutant p53 from natural tumors is altered compared to human p53. Proc. Natl. Acad. Sci. USA 90: 5954-5958, 1993.

Sakaguchi, K., Zambrano, N., Baldwin E. T., Shapiro, B. A., Erickson, J. W., Omichinski, J. G., Clore, G. M., Gronenborn, A. M., and Appella, E.: Identification of a binding site for the human immunodeficiency virus type 1 nucleocapsid protein. Proc. Natl. Acad. Sci. USA 90: 5219-5233, 1993.

Henderson, R. A., Cox, A. L., Sakaguchi, K., Appella, E., Shabanowitz, J., Hunt, D. F, and Engelhard, V. H.: Direct identification of an endogenous peptide recognized by multiple HLA-A2.1-specific cytotoxic T cells. Proc. Natl. Acad. Sci. USA 90: 10275-10279, 1993.

Huczko, E. L., Bonder, W. M., Benjamin, D., Sakaguchi, K., Zhu, N. Z., Shabanowitz, J., Henderson, R. A., Appella, E., Hunt, D. F., and Engelhard, V. H.: Characteristics of endogenous peptides eluted from the class I MHC molecule HLA-B7 determined by mass spectrometry and computer modeling. J. Immunol. 151: 2572-2587, 1993.

Wang, M.-H., Skeel, A., Yoshimura, T., Copeland, T. D., Sakaguchi, K., and Leonard, E. J.: Antibodies to macrophage stimulating protein (MSP): specificity, epitope interactions, and immunoassey of MSP in human seram. J. Leukoc. Biol. 54: 289-295, 1993.

Driscoll, P. C., Altman, J. D., Boniface, J. J., Sakaguchi, K., Reay, P. A., Omichinski, J. G., Appella, E., and Davis, M. M.: Two-dimensional nuclear magnetic resonance analysis of a labeled peptide bound to a class II major histocompatibility complex molecule. J. Mol. Biol. 232: 342-350, 1993.

Matsumoto, H., Shimohigashi, Y., Takano, Y., Sakaguchi, K., Kamiya, H., Ohno, M.: Optimum cross-linking spacer length of dimeric Neurokinin-B analogs for interaction with NK-1 tachykinin receptors. Bull. Chem. Soc. Jpn. 66: 196-204, 1993.

Gao, B., Virmani, M., Romm, E., Lazar-Wesley, E., Sakaguchi, K., Appella, E., Kunos, G., and Takacs, L.: Sequence of cDNA encoding bovine apolipoprotein H. Gene 126: 287-288, 1993.

Fazioli, F., Wong, W. T., Ullrich, S. J., Sakaguchi, K., Appella, E., and Di Fiore, P. P.: The ezrin-like family-of tyrosine kinase substrate: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation. Oncogene 8: 1335-1345, 1993.

Kalia, Y. N., Brocklehurst, S. M., Hipps, D. S., Appella, E., Sakaguchi, K., and Perham, R. N.: The high resolution structure of the peripheral subunit-binding domain of dihydrolipoamide acetyltransferase from the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilis. J. Mol. Biol. 230: 323-341, 1993.

Engelhard, V. H., Appella, E., Benjamin, D. C., Bonder, W. M., Cox, A. L., Chen, Y., Henderson, R. A., Huczko, E. L., Michel, H., Sakaguchi, K., Shabanowitz, J., Sevilir, N., Slingluff, C. L., and Hunt, D. F.: Mass spectrometric analysis of peptides associated with the human class I MHC molecules HLA-A2.1 and HLA-B7 and identification of structural features that determine binding. Chem. Immunol. 57: 39-62, 1993.

Sette, A., Ceman, S., Kubo, R. T., Sakaguchi, K., Appella, E., Hunt, D. F., Davis, T. A., Michel, H., Shabanowitz, J., Rudersdorf, R., Grey, H. M., and DeMars, R.: Invariant chain peptides in most HLA-DR molecules of an antigen processing mutant. Science 258: 1801-1804, 1992.

Bristol, L. A., Sakaguchi, K., Appella, E., Doyle, D., and Takacs, L.: Thymocyte costimulating antigen is CD26 (dipeptidyl-peptidase IV): Costimulation of granulocyte, macrophage and T-lineage cell proliferation via CD26. J. Immunol. 149: 367-372, 1992.

Hunt, D. F., Michel, H., Dickinson, T. A., Shabanowitz, J., Cox, A. L., Sakaguchi, K., Appella, E., Grey, H. M., and Sette, A.: Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-Ad. Science 256: 1817-1820, 1992.

Henderson, R. A.*, Michel, H.*, Sakaguchi, K.*, Shabanowitz, J., Appella, E., Hunt, D. F., and Engelhard, V. H.: HLA-A2.1-associated peptides from a mutant cell line: A second pathway of antigen presentation. Science 255: 1264-1266, 1992. (*co-first author)

Hunt, D. F.*, Henderson, R*. A., Shabanowitz*, J., Sakaguchi, K.*, Michel, H., Sevilir, N., Cox, A. L., Appella, E., and Engelhard, V. H.: Characterization of peptides bound to the class I MHC molecule, HLA-A2.1 by mass spectrometry. Science 255: 1261-1263, 1992. (*co-first author)

Lees-Miller, S. P., Sakaguchi, K., Ullrich, S. J., Appella, E., and Anderson, C. W.: Human DNA-activated protein kinase phosphorylates serines 15 and 37 the amino-terminal transactivation domain of human p53. Mol. Cell. Biol. 12: 5041-5049, 1992.

Omichinski, J. G., Clore, G. M., Robien, M., Sakaguchi, K., Appella, E., and Gronenborn, A. M.: High-resolution solution structure of the double Cys2His2 zinc finger from a human enhancer binding protein MBP-1. Biochemistry 31: 3907-3917, 1992.

Sakaguchi, K., Costa, T., Sakamoto, H., and Shimohigashi, Y.: Receptor interactions of synthetic morphiceptin analogs containing phenylalanine homologs in position 4. Bull. Chem. Soc. Jpn. 65: 1659-1663, 1992.

Olson, D., Pollanen, J., Hoyer-Hansen, G., Ronne, E., Sakaguchi, K., Wen, T.-C., Appella, E., Dano, K., and Blasi, F.: Internalization of the urokinase-plasminogen activator inhibitor type-1 complex is mediated by the urokinase receptor. J. Biol. Chem. 267: 9129-9133, 1992.

Robien, M. A., Clore, G. M., Omichinski, J. G., Perham, R. N., Appella, E., Sakaguchi, K., and Gronenborn, A. M.: Three-dimensional solution structure of the E3-binding domain of the dihydrolipoamide sussinyltransferase core from the 2-oxoglutarate dehydrogenase multienzyme complex of Escherichia coli. Biochemistry 31: 3463-3471, 1992.

Omichinski, J. G., Clore, G. M., Sakaguchi, K., Appella, E., and Gronenborn, A. M.: Structural characterization of a 39-residue synthetic peptide containing the two zinc binding domains from the HIV-1 p7 nucleocapsid protein by CD and NMR spectroscopy. FEBS Lett. 292: 25-30, 1991.

Sakaguchi, K., Appella, E., Omichinski, J. G., Clore, G. M., and Gronenborn, A. M.: Specific DNA binding to an MHC enhancer sequence by a synthetic 57-residue double zinc finger peptide from a human enhancer binding protein. J. Biol. Chem. 266: 7306-7311, 1991.

Demotz, K., Sette, A., Sakaguchi, K., Buchner, R., Appella, E., and Grey, H. D.: Self peptide requirement for Class II major histocompatibility complex allorecognition. Proc. Natl. Acad. Sci. USA 88: 8730-8734, 1991.

Omichinski, J. G., Clore, G. M., Appella, E., Sakaguchi, K., and Gronenborn, A. M.: High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution. Biochemistry 29: 9324-9334, 1990.

Saito, R., Konishi, H., Takano, Y., Nonaka, S., Sakaguchi, K., Shimohigashi, Y., and Kamiya, H.: Characterization of tachykinin receptors in endothelial cells of porcine artery. Neuroscience Lett. 110: 337-342, 1990.

Sakaguchi, K., Sakamoto, H., Tsubaki, Y., Waki, M., Costa, T., and Shimohigashi, Y.: Opioid activities of morphiceptin analogs derived from ?-casein. Bull. Chem. Soc. Jpn. 63: 1753-1757, 1990.

Shimohigashi, Y., Sakaguchi, K., Sakamoto, H., Waki, M., and Costa, T.: Opioid activities of morphiceptin-like peptide latent in various natural protein. Peptide Research 3: 216-223, 1990.

Sakaguchi, K., Kodama, H., Matsumoto, H., Yoshida, M. Takano, Y., Kamiya, H., Waki, M., and Shimohigashi, Y.: Design and synthesis of dimeric analogs of neurokinin A and B: effect of dimerization of COOH-terminal heptapeptides on receptor selection. Peptide Research 2: 345-351, 1989.

Higuchi, Y., Takano, Y., Shimazaki, H., Shimohigashi, Y., Kodama, H., Matsumoto, H., Sakaguchi, K., Nonaka, S., Saito, R., Waki, M., and Kamiya, H.: Dimeric substance P analogue (D-SP2-11) shows a highly potent activity of the in vivo salivary secretion of rat. Eur. J. Pharmacol. 160: 413-416, 1989.

Imazu, S., Shimohigashi, Y., Kodama, H., Sakaguchi, K., Waki, M., Kato., T., and Izumiya, N.: Conformationally stabilized gramicidin S analog containing dehydrophenylalanine in place of D-phenylalanine4,4’: synthesis and antimicrobial activity. Int. J. Pept. Protein Res. 32: 298-306, 1988.

Kodama, H., Shimohigashi, Y., Sakaguchi, K., Waki, M., Takano, Y., Yamada, A., Hatae, Y., and Kamiya, H.: Dimerization of neurokinin A and B COOH-terminal heptapeptide fragments enhanced selectivity for tachykinin receptor subtypes. Eur.J. Pharmacol. 151: 317-320, 1988.

Shimohigashi, Y., Kodama, H., Imazu, S. , Sakaguchi, K., Waki, M., Uchida, H., Kondo, M., and Izumiya, N.: 4,4’-(Z)-dehydrophenyl-alanine]gramicidin S with stabilized bioactive conformation and strong antimicrobial activity. FEBS Lett. 222: 251-255, 1987.