{"id":19,"date":"2014-03-22T11:10:17","date_gmt":"2014-03-22T02:10:17","guid":{"rendered":"http:\/\/barato.sci.hokudai.ac.jp\/~stchem\/?page_id=19"},"modified":"2022-08-06T16:06:57","modified_gmt":"2022-08-06T07:06:57","slug":"pickup","status":"publish","type":"page","link":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/pickup\/","title":{"rendered":"\u767a\u8868\u8ad6\u6587\u304b\u3089pickup"},"content":{"rendered":"<div><div><h2><a href=\"https:\/\/doi.org\/10.1039\/D2DT02137D\">Converting cytochrome <em>c <\/em>into a DyP-like metalloenzyme, Issei Omura, Koichiro Ishimori and Takeshi Uchida, <em>Dalton Trans<\/em>., in press<\/a><\/h2><\/div><p>DyP\u578b\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc(DyP)\u306f\u3001\u30d8\u30e0\u3068\u904e\u9178\u5316\u6c34\u7d20\u306e\u53cd\u5fdc\u3067\u751f\u3058\u305f\u30e9\u30b8\u30ab\u30eb\u306b\u3088\u308a\u3001\u96e3\u5206\u89e3\u6027\u306e\u30a2\u30f3\u30c8\u30e9\u30ad\u30ce\u30f3\u7cfb\u8272\u7d20\u3092\u5bb9\u6613\u306b\u5206\u89e3\u3059\u308b\u3002\u305d\u306e\u305f\u3081\u3001\u3053\u306eDyP\u3092\u767a\u73fe\u3055\u305b\u305f\u5927\u8178\u83cc\u306b\u3088\u308b\u8272\u7d20\u5206\u89e3\u3092\u671f\u5f85\u3057\u305f\u304c\u3001\u5927\u8178\u83cc\u5185\u3067\u306fDyP\u304c\u30d8\u30e0\u3092\u6301\u305f\u306a\u3044\u72b6\u614b\u3067\u767a\u73fe\u3059\u308b\u3053\u3068\u304c\u554f\u984c\u3068\u306a\u3063\u3066\u3044\u305f\u3002\u305d\u3053\u3067\u3001\u751f\u4f53\u5185\u3067\u30d8\u30e0\u3068\u5171\u6709\u7d50\u5408\u3092\u5f62\u6210\u3057\u3066\u767a\u73fe\u3059\u308b\u3001\u96fb\u5b50\u4f1d\u9054\u30bf\u30f3\u30d1\u30af\u8cea\u30b7\u30c8\u30af\u30ed\u30e0<em>c<\/em> (cyt<em> c<\/em>)\u3092\u7528\u3044\u3001\u5927\u8178\u83cc\u5185\u3067\u52b9\u7387\u7684\u306b\u96e3\u5206\u89e3\u6027\u8272\u7d20\u3092\u5206\u89e3\u53ef\u80fd\u306a\u9175\u7d20\u306e\u8a2d\u8a08\u3092\u8a66\u307f\u305f\u3002DyP\u306e\u69cb\u9020\u306b\u898b\u3089\u308c\u308b\u30d8\u30e0\u8fd1\u4f4d\u5074\u306e\u6c34\u7d20\u7d50\u5408\u3092\u5c0e\u5165\u3057\u3001\u8272\u7d20\u5206\u89e3\u306b\u5fc5\u9808\u306a\u30e9\u30b8\u30ab\u30eb\u306e\u751f\u6210\u3092\u4fc3\u9032\u3059\u308b\u305f\u3081\u3001cyt <em>c<\/em>\u306eGly29\u3092Asp\u306b\u7f6e\u63db\u3057\u305f\u7d50\u679c\u3001\u4e2d\u6027\u6761\u4ef6\u3067\u91ce\u751f\u578b\u306e\u8272\u7d20\u5206\u89e3\u6d3b\u6027\u306e\u7d0480\u500d\u306e\u89e6\u5a92\u52b9\u7387\u3092\u793a\u3059\u5909\u7570\u4f53\u306e\u4f5c\u6210\u306b\u6210\u529f\u3057\u305f\u3002\u3053\u306e\u7d50\u679c\u306fDyP\u3092\u6a21\u5023\u3057\u305f\u8272\u7d20\u5206\u89e3\u9175\u7d20\u306e\u8a2d\u8a08\u306b\u65b0\u305f\u306a\u77e5\u898b\u3092\u4e0e\u3048\u308b\u3082\u306e\u3067\u3042\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC.png\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1792 aligncenter colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC.png\" alt=\"\" width=\"352\" height=\"201\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC.png 913w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-300x172.png 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-768x439.png 768w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-690x395.png 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-540x309.png 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-340x194.png 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-223x127.png 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/08\/TOC-124x71.png 124w\" sizes=\"(max-width: 352px) 100vw, 352px\" \/><\/a><\/p><div><h2><a href=\"https:\/\/doi.org\/10.1016\/j.jinorgbio.2022.111713\">Regulation of the expression of the nickel uptake system in Vibrio cholerae by iron and heme via ferric uptake regulator (Fur), Kazuyoshi Muranishi, Koichiro Ishimori and Takeshi Uchida, J. Inorg. Biochem. 298, 11713 (2022)<\/a><\/h2><p>\u30b3\u30ec\u30e9\u83cc\u3067\u306f\u3001\u30cb\u30c3\u30b1\u30eb\u306e\u53d6\u308a\u8fbc\u307f\u306b\u95a2\u4e0e\u3059\u308b\u907a\u4f1d\u5b50&#8221;<em>nik<\/em>\u30aa\u30da\u30ed\u30f3&#8221;\u306e\u4e0a\u6d41\u306b\u3001\u9244\u5fdc\u7b54\u6027\u306e\u8ee2\u5199\u5236\u5fa1\u56e0\u5b50\u3067\u3042\u308bFur\u304c\u5b58\u5728\u3059\u308b\u3002\u5927\u8178\u83cc\u306a\u3069\u306e\u4ed6\u306e\u751f\u7269\u7a2e\u3067\u306f\u3001<em>nik<\/em>\u30aa\u30da\u30ed\u30f3\u306f\u7d30\u80de\u5185\u306e\u30cb\u30c3\u30b1\u30eb\u306e\u6fc3\u5ea6\u4f9d\u5b58\u7684\u306b\u8ee2\u5199\u304c\u8abf\u7bc0\u3055\u308c\u308b\u3001\u30b3\u30ec\u30e9\u83cc\u3068\u306f\u7570\u306a\u308b\u8ee2\u5199\u5236\u5fa1\u6a5f\u69cb\u3092\u6301\u3064\u3002\u3053\u308c\u307e\u3067\u30cb\u30c3\u30b1\u30eb\u304cFur\u306b\u3088\u308b\u907a\u4f1d\u5b50\u8ee2\u5199\u5236\u5fa1\u306b\u95a2\u308f\u308b\u3068\u3044\u3046\u5831\u544a\u306f\u306a\u304b\u3063\u305f\u3053\u3068\u304b\u3089\u3001\u30b3\u30ec\u30e9\u83cc\u306e<em>nik<\/em>\u30aa\u30da\u30ed\u30f3\u304cFur\u3092\u4ecb\u3057\u3066\u3001\u30cb\u30c3\u30b1\u30eb\u3067\u5236\u5fa1\u3055\u308c\u308b\u306e\u304b\u9244\u3067\u5236\u5fa1\u3055\u308c\u308b\u306e\u304b\u3092\u691c\u8a0e\u3057\u305f\u3002<\/p><p>Fur\u306fFe<sup>2+<\/sup>\u306e\u5b58\u5728\u4e0b\u3067Fur box\u3068\u547c\u3070\u308c\u308b\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u914d\u5217\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001Ni<sup>2+<\/sup>\u5b58\u5728\u4e0b\u3067\u306fDNA\u3068Fur\u304c\u975e\u7279\u7570\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u3057\u305f\u3002\u307e\u305f\u3001\u7d30\u80de\u5185\u306e\u9244\u306e\u307b\u3068\u3093\u3069\u306f\u30d8\u30e0\u3068\u3057\u3066\u5b58\u5728\u3059\u308b\u3053\u3068\u304b\u3089\u3001\u30d8\u30e0\u306b\u3088\u308bFur\u306eFur box\u7d50\u5408\u3078\u306e\u5f71\u97ff\u3092\u691c\u8a0e\u3057\u305f\u3068\u3053\u308d\u3001\u30d8\u30e0\u306fFur-Fur box\u8907\u5408\u4f53\u3078\u306e\u30d8\u30e0\u6dfb\u52a0\u306f\u3001Fur box\u304b\u3089\u306eFur\u306e\u89e3\u96e2\u3092\u8a98\u767a\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001\u30b3\u30ec\u30e9\u83cc\u306e<em>nik<\/em>\u30aa\u30da\u30ed\u30f3\u306f\u9244\u3068\u30d8\u30e0\u306e\u4e21\u65b9\u306b\u3088\u3063\u3066\u767a\u73fe\u304c\u8abf\u7bc0\u3055\u308c\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u3055\u3089\u306b\u3001<em>nik<\/em>\u30aa\u30da\u30ed\u30f3\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308bVCA1098\u306f\u30011.3\u03bcM\u306e\u89e3\u96e2\u5b9a\u6570\u3067\u30d8\u30e0\u306b\u7d50\u5408\u3057\u305f\u3002\u6211\u3005\u306e\u7d50\u679c\u306f\u3001<em>nik<\/em>\u30aa\u30da\u30ed\u30f3\u304c\u30cb\u30c3\u30b1\u30eb\u306e\u53d6\u308a\u8fbc\u307f\u3060\u3051\u3067\u306a\u304f\u30d8\u30e0\u306e\u53d6\u308a\u8fbc\u307f\u306b\u3082\u95a2\u4e0e\u3057\u3066\u304a\u308a\u3001\u7d30\u83cc\u5185\u306e\u9244\u3068\u30d8\u30e0\u306e\u6fc3\u5ea6\u306b\u4f9d\u5b58\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1758 aligncenter colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-1024x411.jpg\" alt=\"\" width=\"566\" height=\"227\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-1024x411.jpg 1024w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-300x120.jpg 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-768x308.jpg 768w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-690x277.jpg 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-540x217.jpg 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-340x136.jpg 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-223x89.jpg 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg-124x50.jpg 124w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2022\/03\/1-s2.0-S0162013422000022-ga1_lrg.jpg 1324w\" sizes=\"(max-width: 566px) 100vw, 566px\" \/><\/a><\/p><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0162013421000696\">Radical transfer but not heme distal residues is essential for pH dependence of dye-decolorizing activity of peroxidase from <em>Vibrio cholerae<\/em>, Takeshi Uchida, Issei Omura, Sayaka Umetsu and Koichiro Ishimori, J Inorg. Biochem., in press<\/a><\/h2><p>\u8272\u7d20\u5206\u89e3\u578b\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\uff08DyP\uff09\u306f\u3001\u904e\u9178\u5316\u6c34\u7d20\u3092\u7528\u3044\u3066\u30a2\u30f3\u30c8\u30e9\u30ad\u30ce\u30f3\u7cfb\u8272\u7d20\u306e\u5206\u89e3\u3092\u89e6\u5a92\u3059\u308b\u30d8\u30e0\u9175\u7d20\u3067\u3042\u308b\u3002DyP\u306e\u8272\u7d20\u5206\u89e3\u53cd\u5fdc\u306b\u306fpH\u4f9d\u5b58\u6027\u304c\u3042\u308a\u3001\u9178\u6027\u57df\u3067\u306e\u307f\u9032\u884c\u3059\u308b\u304c\u305d\u306e\u8981\u56e0\u306f\u4e0d\u660e\u3067\u3042\u3063\u305f\u305f\u3081\u3001\u30b3\u30ec\u30e9\u83cc\u7531\u6765DyP(<em>Vc<\/em>DyP)\u306e\u5909\u7570\u4f53\u3092\u3082\u3061\u3044\u3066\u691c\u8a0e\u3057\u305f\u3002DyP\u306f\u3001\u30d8\u30e0\u3068\u904e\u9178\u5316\u6c34\u7d20\u306e\u53cd\u5fdc\u306b\u3088\u308a\u30e9\u30b8\u30ab\u30eb\u304c\u751f\u3058\u3001\u751f\u6210\u3055\u308c\u305f\u30e9\u30b8\u30ab\u30eb\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u8868\u9762\u306e\u6d3b\u6027\u90e8\u4f4d\u306b\u79fb\u52d5\u3059\u308b\u3053\u3068\u3067\u8272\u7d20\u3092\u5206\u89e3\u3059\u308b\u3002<em>Vc<\/em>DyP\u306e\u30d8\u30e0\u9060\u4f4d\u5074\u306b\u4f4d\u7f6e\u3059\u308bAsp144\u306f\u30e9\u30b8\u30ab\u30eb\u751f\u6210\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046\u6b8b\u57fa\u3067\u3042\u308a\u3001\u3053\u306eAsp144\u3092His\u306b\u7f6e\u63db\u3059\u308b\u3068\u6d3b\u6027\u304c\u5931\u308f\u308c\u305f\u3002\u3053\u306eAsp144\u3088\u308a\u3082\u30d8\u30e0\u304b\u3089\u96e2\u308c\u305f\u4f4d\u7f6e\u306bHis\u3092\u5c0e\u5165\u3057\u305f\u5909\u7570\u4f53\u3092\u4f5c\u6210\u3057\u305f\u3068\u3053\u308d\u3001\u6d3b\u6027\u306f\u56de\u5fa9\u3057\u305f\u304c\u8272\u7d20\u5206\u89e3\u6d3b\u6027\u306e\u81f3\u9069pH\u306f\u9178\u6027\u57df\u306e\u307e\u307e\u3067\u3042\u3063\u305f\u3053\u3068\u304b\u3089\u3001Asp144\u306f\u8272\u7d20\u5206\u89e3\u53cd\u5fdc\u306epH\u4f9d\u5b58\u6027\u306b\u5bc4\u4e0e\u3057\u306a\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u305d\u3053\u3067\u3001\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u89e3\u6790\u306b\u3088\u308a\u30e9\u30b8\u30ab\u30eb\u306e\u79fb\u52d5\u7d4c\u8def\u3092\u63a2\u7d22\u3057\u305f\u7d50\u679c\u3001\u305d\u306e\u7d4c\u8def\u4e0a\u306b\u4f4d\u7f6e\u3059\u308bAsp138\u306fHis178\u3068\u6c34\u7d20\u7d50\u5408\u53ef\u80fd\u306a\u4f4d\u7f6e\u306b\u5b58\u5728\u3057\u3066\u3044\u305f\u3002\u3053\u306eAsp138\u3092Val\u306b\u7f6e\u63db\u3057\u3001\u6c34\u7d20\u7d50\u5408\u5f62\u6210\u3067\u304d\u306a\u3044\u5909\u7570\u4f53\u3092\u4f5c\u6210\u3057\u305f\u3068\u3053\u308d\u3001\u8272\u7d20\u5206\u89e3\u6d3b\u6027\u306e\u81f3\u9069pH\u304c\u4e2d\u6027\u57df\u306b\u79fb\u52d5\u3057\u305f\u3002\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089\u3001<em>Vc<\/em>DyP\u306e\u8272\u7d20\u5206\u89e3\u53cd\u5fdc\u306epH\u4f9d\u5b58\u6027\u306b\u306f\u3001\u30d8\u30e0\u8fd1\u508d\u306eAsp138\u3068His178\u9593\u306e\u6c34\u7d20\u7d50\u5408\u304c\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter  wp-image-1635 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg.jpg\" alt=\"\" width=\"283\" height=\"169\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg.jpg 1484w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-300x179.jpg 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-768x459.jpg 768w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-1024x611.jpg 1024w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-690x412.jpg 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-540x322.jpg 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-340x203.jpg 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-223x133.jpg 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2021\/03\/1-s2.0-S0162013421000696-ga1_lrg-124x74.jpg 124w\" sizes=\"(max-width: 283px) 100vw, 283px\" \/><\/a><\/p><p>&nbsp;<\/p><\/div><h2><a href=\"https:\/\/www.jbc.org\/content\/295\/32\/11316\">Mechanistic insights into heme-mediated transcriptional regulation via a bacterial manganese-binding iron regulator, iron response regulator (Irr), Dayeon Nam, Yuki Matsumoto, Takeshi Uchida, Mark R. O&#8217;Brian and Koichiro Ishimori, J. Biol. Chem., 295, 11316-11325 (2020) (DOI: 10.1074\/jbc.RA119.011855)<\/a><\/h2><p>\u7a92\u7d20\u56fa\u5b9a\u83cc\uff08Bradyrhizobium japonicum\uff09\u306e\u30d8\u30e0\u751f\u5408\u6210\u3092\u8ee2\u5199\u30ec\u30d9\u30eb\u3067\u6291\u5236\u7684\u306b\u5236\u5fa1\u3059\u308b\u8ee2\u5199\u56e0\u5b50Iron Response Regulator\uff08Irr\uff09\u306f\u3001\u7d30\u80de\u5185\u3067\u5229\u7528\u3067\u304d\u308b\u9244\u91cf\u304c\u5341\u5206\u3067\u3042\u308b\u3068\u3001\u30d8\u30e0\u306e\u7d50\u5408\u306b\u3088\u308a\u9178\u5316\u7684\u4fee\u98fe\u304c\u8a98\u8d77\u3055\u308c\u3001\u305d\u306e\u7d50\u679c\u3001Irr\u305d\u306e\u3082\u306e\u304c\u5206\u89e3\u3055\u308c\u308b\u3053\u3068\u3067\u6a19\u7684DNA\u914d\u5217\u3067\u3042\u308bIron Control Element\uff08ICE\uff09\u304b\u3089\u89e3\u96e2\u3057\u3001\u30d8\u30e0\u751f\u5408\u6210\u304c\u9032\u884c\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u305f\u3002\u3057\u304b\u3057\u3001\u4eca\u56de\u306e\u8ad6\u6587\u3067\u306f\u3001Irr\u306eICE\u3078\u306e\u7d50\u5408\u306fMn2+\u306e\u7d50\u5408\u304c\u5fc5\u9808\u3067\u3042\u308a\u3001\u30d8\u30e0\u306e\u7d50\u5408\u306b\u3088\u308aMn2+\u304c\u89e3\u96e2\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001Irr\u306f\u9178\u5316\u4fee\u98fe\u3092\u53d7\u3051\u308b\u3053\u3068\u306a\u304fICE\u3078\u306e\u7d50\u5408\u80fd\u3092\u5931\u3046\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u306f\u3001Irr\u306b\u304a\u3051\u308b\u30d8\u30e0\u306b\u4f9d\u5b58\u3057\u305f\u65b0\u305f\u306a\u8ee2\u5199\u5236\u5fa1\u6a5f\u69cb\u3092\u63d0\u6848\u3059\u308b\u3068\u3068\u3082\u306b\u3001\u7d30\u80de\u5185\u3067\u306e\u751f\u547d\u91d1\u5c5e\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u3082\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1567 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31.jpg\" alt=\"\" width=\"567\" height=\"113\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31.jpg 567w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-300x60.jpg 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-540x108.jpg 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-340x68.jpg 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-223x44.jpg 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/\u30d7\u30ec\u30bc\u30f3\u30c6\u30fc\u30b7\u30e7\u30f31-124x25.jpg 124w\" sizes=\"(max-width: 567px) 100vw, 567px\" \/><\/a><\/p><h2><a href=\"https:\/\/portlandpress.com\/biochemj\/article\/477\/8\/1565\/222579\/Osmotic-pressure-effects-identify-dehydration-upon\">Osmotic pressure effects identify dehydration upon cytochrome <em>c<\/em>\u2013cytochrome <em>c<\/em> oxidase complex formation contributing to a specific electron pathway formation,\u00a0Wataru Sato, Seiji Hitaoka, Takeshi Uchida,\u00a0Kyoko Shinzawa-Itoh, Kazunari Yoshizawa, Shinya Yoshikawa and\u00a0 Koichiro Ishimori,\u00a0Biochem. J., 477,\u00a0 <em>Biochem J<\/em>\u00a0(2020) 477, 1565\u20131578 (2020)\u00a0(DOI: 10.1042\/BCJ20200023)<\/a><\/h2><p>ATP\u5408\u6210\u306e\u4e2d\u67a2\u3092\u62c5\u3046\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u96fb\u5b50\u4f1d\u9054\u7cfb\u306b\u304a\u3044\u3066\u3001\u30d8\u30e0\u30bf\u30f3\u30d1\u30af\u8cea\u30b7\u30c8\u30af\u30ed\u30e0c(Cyt c)\u306f\u8907\u5408\u4f53III\u304b\u3089\u30b7\u30c8\u30af\u30ed\u30e0c\u9178\u5316\u9175\u7d20(CcO)\u3078\u306e\u96fb\u5b50\u79fb\u52d5\u3092\u4ef2\u4ecb\u3059\u308b\u5f79\u5272\u3092\u6709\u3059\u308b\u3002\u3053\u308c\u3089Cyt c-CcO\u9593\u3067\u5f62\u6210\u3055\u308c\u308b\u96fb\u5b50\u4f1d\u9054\u8907\u5408\u4f53\u306f\u3001\u8fd1\u5e74\u306e\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3(Sato et al., J. Biol. Chem., 2016)\u3088\u308a\u3001\u5f93\u6765\u63d0\u5531\u3055\u308c\u3066\u304d\u305f\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\u3088\u308a\u3082\u3080\u3057\u308d\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306e\u5f62\u6210\u306b\u3088\u3063\u3066\u5b89\u5b9a\u5316\u3055\u308c\u308b\u3053\u3068\u304c\u4e88\u6e2c\u3055\u308c\u3066\u3044\u305f\u3002\u672c\u7814\u7a76\u3067\u306f\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306e\u5f62\u6210\u306b\u4f34\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u758e\u6c34\u9762\u306e\u6c34\u5206\u5b50\u304c\u653e\u51fa\uff08\u8131\u6c34\u548c\uff09\u3055\u308c\u308b\u3053\u3068\u306b\u7740\u76ee\u3057\u3001\u3053\u306e\u8131\u6c34\u548c\u904e\u7a0b\u3092Cyt c-CcO\u9593\u96fb\u5b50\u4f1d\u9054\u53cd\u5fdc\u306b\u304a\u3051\u308b\u6d78\u900f\u5727\u52b9\u679c\u3092\u901a\u3057\u3066\u691c\u8a0e\u3059\u308b\u3053\u3068\u3067\u3001\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306e\u8907\u5408\u4f53\u5f62\u6210\u3078\u306e\u71b1\u529b\u5b66\u7684\u5bc4\u4e0e\u3092\u8003\u5bdf\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001\u3053\u308c\u3089\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u3067\u8907\u5408\u4f53\u3092\u5f62\u6210\u3059\u308b\u969b\u306b\u306f20\u5206\u5b50\u7a0b\u5ea6\u306e\u6c34\u5206\u5b50\u304c\u8131\u6c34\u548c\u3057\u3066\u304a\u308a\u3001\u3053\u306e\u8131\u6c34\u548c\u306b\u8d77\u56e0\u3059\u308b\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u306e\u5897\u5927\u304c\u4f1a\u5408\u306b\u4f34\u3046\u4e26\u9032\u56de\u8ee2\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u306e\u6e1b\u5c11\u3092\u88dc\u511f\u3057\u3001\u8907\u5408\u4f53\u306e\u5b89\u5b9a\u5316\u306b\u5bc4\u4e0e\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u3055\u3089\u306b\u3001\u5909\u7570\u4f53\u5b9f\u9a13\u53ca\u3073\u30c9\u30c3\u30ad\u30f3\u30b0\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3088\u308a\u3001\u8131\u6c34\u548c\u3057\u305f\u6c34\u5206\u5b50\u306e\u7d04\u534a\u6570\u304cCyt c\u306e\u30d8\u30e0\u8fd1\u508d\u306b\u4f4d\u7f6e\u3059\u308b\u758e\u6c34\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57faIle81\u5468\u8fba\u306b\u7531\u6765\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u65b0\u305f\u306b\u793a\u3055\u308c\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u30d8\u30e0\u8fd1\u508d\u3067\u5f62\u6210\u3055\u308c\u308b\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306f\u9178\u5316\u9084\u5143\u4e2d\u5fc3\u9593\u3078\u306e\u6c34\u5206\u5b50\u306e\u6d41\u5165\u3092\u9632\u3050\u3068\u3044\u3046\u70b9\u3067\u3001Cyt c-CcO\u9593\u306e\u96fb\u5b50\u4f1d\u9054\u7d4c\u8def\u306e\u5f62\u6210\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u63a8\u5bdf\u3055\u308c\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/20200407-BJ_graphical_abstract-002-e1600296086235.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1570 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/20200407-BJ_graphical_abstract-002-e1600296086235.png\" alt=\"\" width=\"160\" height=\"150\" \/><\/a><\/p><h2><a href=\"https:\/\/www.nature.com\/articles\/s41598-019-52766-6\">Quantitative description and classification of protein structures by a novel robust amino acid network: interaction selective network (ISN), Shohei Konno, Takao Namiki and Koichiro Ishimori,\u00a0Sci. Rep., 9, 16654 (2019)\u00a0(DOI: 10.1038\/s41598-019-52766-6)<\/a><\/h2><p>\u30bf\u30f3\u30d1\u30af\u8cea\u306f\uff0c\u30a2\u30df\u30ce\u9178\u304c\u76f4\u9396\u72b6\u306b\u3064\u306a\u304c\u3063\u305f\u30da\u30d7\u30c1\u30c9\u304c\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u308a\u4e00\u5b9a\u306e\u69cb\u9020\u3092\u5f62\u6210\u3057\u3066\u6a5f\u80fd\u3057\u3066\u304a\u308a\uff0c\u3059\u3079\u3066\u306e\u751f\u7269\u306e\u751f\u547d\u6d3b\u52d5\u3092\u62c5\u3046\u91cd\u8981\u306a\u5206\u5b50\u3067\u3059\u3002\u3064\u307e\u308a\uff0c\u751f\u547d\u6d3b\u52d5\u3092\u30df\u30af\u30ed\u306a\u76ee\u3067\u89e3\u6790\u3059\u308b\u305f\u3081\u306b\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u3092\u77e5\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\uff0c\u3053\u308c\u307e\u3067\u591a\u304f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u304c\u89e3\u660e\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u69cb\u9020\u306f\u591a\u69d8\u3067\u3059\u304c\uff0c\u90e8\u5206\u7684\u306a\u69cb\u9020\u306f\u985e\u4f3c\u3057\u3066\u3044\u308b\u5834\u5408\u304c\u591a\u3044\u305f\u3081\u591a\u304f\u306e\u7814\u7a76\u8005\u304c\u4e00\u5b9a\u306e\u5206\u985e\u3092\u3067\u304d\u308b\u3068\u8003\u3048\u3066\u304d\u307e\u3057\u305f\u3002\u3057\u304b\u3057\uff0c\u305d\u306e\u5206\u985e\u57fa\u6e96\u306f\u7814\u7a76\u8005\u306e\u4e3b\u89b3\u306b\u983c\u308b\u3068\u3053\u308d\u304c\u591a\u304f\uff0c\u307e\u305f\u5ba2\u89b3\u7684\u306a\u89e3\u6790\u304c\u671f\u5f85\u3067\u304d\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u69cb\u9020\u306e\u6570\u5b66\u7684\u89e3\u6790\u3067\u3082\u5206\u985e\u306b\u6210\u529f\u3057\u3066\u3044\u307e\u305b\u3093\u3002\u3053\u306e\u3088\u3046\u306b\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u69cb\u9020\u304c\u6a5f\u80fd\u3092\u6c7a\u3081\uff0c\u305d\u308c\u306b\u3088\u3063\u3066\u751f\u547d\u6d3b\u52d5\u3092\u7dad\u6301\u3057\u3066\u3044\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u69cb\u9020\u3092\u5ba2\u89b3\u7684\u306a\u6307\u6a19\u306b\u57fa\u3065\u3044\u3066\u5206\u985e\u3059\u308b\u3053\u3068\u306f\u56f0\u96e3\u3067\u3057\u305f\u3002<br \/>\u3053\u306e\u8ad6\u6587\u3067\u306f\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u69cb\u6210\u3059\u308b\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u70b9\u3067\uff0c\u7d50\u5408\u3084\u76f8\u4e92\u4f5c\u7528\u3057\u3066\u3044\u308b\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u70b9\u3068\u70b9\u3092\u3064\u306a\u3050\u7dda\u3068\u3057\u3066\u6349\u3048\u308b\u65b0\u305f\u306a\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u7406\u8ad6\u3092\u69cb\u7bc9\u3057\uff0c\u305d\u306e\u7406\u8ad6\u3092\u5b9f\u969b\u306e\u7d041,500\u7a2e\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u69cb\u9020\u306b\u5bfe\u3057\u3066\u9069\u7528\u3057\u307e\u3057\u305f\u3002<br \/>\u305d\u306e\u7d50\u679c\uff0c\u7279\u5b9a\u306e\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u7acb\u4f53\u69cb\u9020\u306e\u5206\u985e\u306b\u6210\u529f\u3057\uff0c\u6570\u5b66\u7684\u306a\u5ba2\u89b3\u7684\u6307\u6a19\u306b\u3088\u308a\u30bf\u30f3\u30d1\u30af\u8cea\u5168\u4f53\u306e\u69cb\u9020\u306e\u5206\u985e\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002<br \/>\u3053\u308c\u306b\u3088\u308a\uff0c\u3069\u306e\u3088\u3046\u306a\u69cb\u9020\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u306e\u304b\uff0c\u3042\u308b\u3044\u306f\u751f\u7269\u9032\u5316\u306e\u904e\u7a0b\u3067\u3069\u306e\u3088\u3046\u306a\u69cb\u9020\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u9078\u3070\u308c\u3066\u304d\u305f\u306e\u304b\uff0c\u3055\u3089\u306b\u3069\u306e\u3088\u3046\u306a\u69cb\u9020\u3092\u3082\u305f\u305b\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u4eba\u5de5\u7684\u306b\u5b89\u5b9a\u3067\u9ad8\u6a5f\u80fd\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u985e\u4f3c\u7269\u8cea\u3084\u751f\u4f53\u5206\u5b50\u985e\u4f3c\u7269\u8cea\u3092\u5275\u6210\u3067\u304d\u308b\u306e\u304b\u306a\u3069\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u79d1\u5b66\u306b\u304a\u3051\u308b\u6709\u7528\u306a\u60c5\u5831\u3092\u4e0e\u3048\u308b\u3068\u671f\u5f85\u3055\u308c\u307e\u3059\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/2-e1600297701575.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1576 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/2-e1600297701575.jpg\" alt=\"\" width=\"200\" height=\"150\" \/><\/a><\/p><\/div><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0162013419304969\">A single mutation converts Alr5027 from cyanobacteria Nostoc sp. PCC 7120 to a heme-binding\u00a0protein with heme-degrading ability, Nobuhiko Dojun, Kazuyoshi Muranishi, Koichiro Ishimori and Takeshi Uchida, J. Inorg. Biochem., 203, 110916 (11 pages) (2020) (DOI: 10.1016\/j.jinorgbio.2019.<wbr \/>110916)<\/a><\/h2><p>\u30b7\u30a2\u30ce\u30d0\u30af\u30c6\u30ea\u30a2\u306b\u306f\u30b3\u30ec\u30e9\u83cc\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20HutZ\u3068\u914d\u5217\u304c\u4f3c<wbr \/>\u305fAlr5027\u3068\u3044\u3046\u6a5f\u80fd\u304c\u4e0d\u660e\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u3042\u308b\u3002<wbr \/>\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30d8\u30e0\u3068\u7d50\u5408\u3057\u306a\u3044\u304c\u3001<wbr \/>Lys154\u3092His\u306b\u7f6e\u63db\u3059\u308b\u3060\u3051\u3067\u30d8\u30e0\u3068\u7d50\u5408\u3059\u308b\u3088\u3046\u306b\u306a\u308a<wbr \/>\u3001<wbr \/>\u9178\u5316\u5264\u3067\u3042\u308b\u904e\u9178\u5316\u6c34\u7d20\u3092\u52a0\u3048\u308b\u3068\u30d9\u30eb\u30c9\u30d8\u30e0\u3068\u547c\u3070\u308c\u308b\u30d8\u30e0\u3092\u5206<wbr \/>\u89e3\u3059\u308b\u904e\u7a0b\u3067\u73fe\u308c\u308b\u7279\u5fb4\u7684\u306a\u4e2d\u9593\u4f53\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u3055\u3089\u306b\u3001<wbr \/>\u5f62\u6210\u3057\u305f\u30d9\u30eb\u30c9\u30d8\u30e0\u306b\u30a2\u30b9\u30b3\u30eb\u30d3\u30f3\u9178\u3092\u52a0\u3048\u308b\u3068HutZ\u304c\u30d8\u30e0\u3092<wbr \/>\u5206\u89e3\u3059\u308b\u969b\u306e\u6700\u7d42\u7523\u7269\u3067\u3042\u308b\u30d3\u30ea\u30d9\u30eb\u30b8\u30f3\u3092\u5f62\u6210\u3057\u305f\u3053\u3068\u304b\u3089\u3001<wbr \/>HutZ\u3068\u540c\u3058\u53cd\u5fdc\u904e\u7a0b\u3067\u30d8\u30e0\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>HutZ\u306f\u7acb\u4f53\u69cb\u9020\u304c\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20HO\u3068\u7570\u306a\u308b\u3053\u3068\u304b\u3089H<wbr \/>O\u3068\u7570\u306a\u308b\u7956\u5148\u304b\u3089\u751f\u3058\u305f\u3068\u8003\u3048\u3089\u308c\u308b\u304c\u3001<wbr \/>\u6700\u521d\u306e\u9178\u7d20\u767a\u751f\u578b\u5149\u5408\u6210\u7d30\u83cc\u3067\u3042\u308b\u30b7\u30a2\u30ce\u30d0\u30af\u30c6\u30ea\u30a2\u306b\u305d\u306e\u7956\u5148\u3068<wbr \/>\u306a\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002<a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/11\/JIB-Alr5027.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1481 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/11\/JIB-Alr5027-150x150.png\" alt=\"\" width=\"150\" height=\"150\" \/><\/a><\/p><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0162013419301242\">Specific heme binding to heme regulatory motifs in iron regulatory proteins and its functional significance,Yudai Nishitani, Hirotaka Okutani, Yukiko Takeda, Takeshi Uchida, Kazuhiro Iwai, Koichiro Ishimori 5 , J. Inorg. Biochem., 198, 110726203 (2019) (DOI: 10.1016\/j.jinorgbio.2019.110726)<\/a><\/h2><p>\u9244\u306f\u751f\u4f53\u5185\u3067\u69d8\u3005\u306a\u6a5f\u80fd\u3092\u6709\u3057\u3066\u3044\u308b\u300c\u751f\u4f53\u91d1\u5c5e\u300d\u3067\u3001\u3059\u3079\u3066\u306e\u751f\u7269\u306b\u3068\u3063\u3066\u5fc5\u9808\u306e\u9077\u79fb\u5143\u7d20\u3067\u3042\u308b\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u7d30\u80de\u5185\u3067\u306e\u9244\u4e0d\u8db3\u306f\u591a\u304f\u306e\u9244\u542b\u6709\u86cb\u767d\u8cea\u306e\u6a5f\u80fd\u4e0d\u5168\u3084\u3001\u75c5\u614b\u3068\u3057\u3066\u306e\u8ca7\u8840\u3092\u5f15\u304d\u8d77\u3053\u3059\u3002\u4e00\u65b9\u3001\u904e\u5270\u306a\u9244\u3082\u7d30\u80de\u5185\u3067\u6d3b\u6027\u9178\u7d20\u3092\u7523\u751f\u3057\u3001\u7a2e\u3005\u306e\u7d30\u80de\u5185\u7269\u8cea\u3092\u640d\u50b7\u3057\u3001\u809d\u786c\u5909\u3084\u795e\u7d4c\u5909\u6027\u75be\u60a3\u3092\u5f15\u304d\u8d77\u3053\u3059\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002\u3053\u306e\u3088\u3046\u306a\u9244\u306e\u7d30\u80de\u5185\u3067\u306e\u6052\u5e38\u6027\u3092\u7dad\u6301\u3059\u308b\u306e\u304c\u9244\u5236\u5fa1\u86cb\u767d\u8cea\u3001Iron Response Regulator\uff08IRP\uff09\u3067\u3042\u308a\u3001\u3053\u306eIRP\u306f\u7d30\u80de\u5185\u3078\u9244\u3092\u53d6\u308a\u8fbc\u307f\u3001\u3042\u308b\u3044\u306f\u8caf\u8535\u3059\u308b\u86cb\u767d\u8cea\u306e\u7ffb\u8a33\u3092\u5236\u5fa1\u3059\u308bRNA\u7d50\u5408\u5236\u5fa1\u86cb\u767d\u8cea\u3067\u3042\u308b\u3002\u3053\u308c\u307e\u3067\u306e\u6211\u3005\u306e\u7814\u7a76\u304b\u3089\u3001IRP\u306e2\u3064\u306e\u76f8\u540c\u4f53\u3067\u3042\u308bIRP1\u3001IRP2\u3068\u3082\u9244\u30dd\u30eb\u30d5\u30a3\u30ea\u30f3\u932f\u4f53\u3067\u3042\u308b\u30d8\u30e0\u3092\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u5206\u5149\u5b66\u7684\u306b\u793a\u3055\u308c\uff08Ogura et al., J. Inorg. Biochem., 2018, 182, 238-248\uff09\u3066\u304d\u305f\u304c\u3001\u305d\u306e\u6a5f\u80fd\u7684\u610f\u7fa9\u306f\u4e0d\u660e\u306a\u70b9\u304c\u591a\u304b\u3063\u305f\u3002\u672c\u7814\u7a76\u3067\u306f\u3001Native-PAGE\u6cd5\u3068Oxyblot\u6cd5\u3068\u3044\u3063\u305f\u751f\u5316\u5b66\u7684\u624b\u6cd5\u3092\u7528\u3044\u3066\u3001IRP1\u3001IRP2\u3068\u3082\u30d8\u30e0\u3092\u305d\u306e\u30d8\u30e0\u7d50\u5408\u30e2\u30c1\u30fc\u30d5\uff08Heme Regulatory Motif\uff09\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3057\u3001\u305d\u306e\u30d8\u30e0\u7d50\u5408\u306f\u3044\u305a\u308c\u306eIRP\u306b\u5bfe\u3057\u3066\u3082\u305d\u306e\u7d50\u5408RNA\u914d\u5217\u3067\u3042\u308b\u9244\u5fdc\u7b54\u8981\u7d20\uff08Iron Responsive Element: IRE\uff09\u3078\u306e\u7d50\u5408\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u3001\u307e\u305f\u3001IRE\u3092\u7d50\u5408\u3057\u305fIRP1\u3001IRP2\u306b\u30d8\u30e0\u3092\u6dfb\u52a0\u3059\u308b\u3068IRE\u304b\u3089\u306e\u89e3\u96e2\u304c\u89b3\u6e2c\u3055\u308c\u305f\u3002\u3055\u3089\u306b\u3001IRP2\u306e\u5834\u5408\u306b\u306f\u3001IRP1\u306b\u306f\u5b58\u5728\u3057\u306a\u3044\u633f\u5165\u914d\u5217\u3001\u9244\u4f9d\u5b58\u6027\u5206\u89e3\uff08Iron-dependent Degradation: IDD\uff09\u30c9\u30e1\u30a4\u30f3\u306b\u7d50\u5408\u3057\u305f\u30d8\u30e0\u306f\u3001\u86cb\u767d\u8cea\u90e8\u5206\u306e\u9178\u5316\u4fee\u98fe\u3092\u5f15\u304d\u8d77\u3053\u3057\u3001IRP2\u306e\u5206\u89e3\u304c\u9032\u884c\u3059\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u306f\u3001IRP\u306eIRE\u7d50\u5408\u3092\u30d8\u30e0\u304c\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u5185\u3067\u5229\u7528\u3067\u304d\u308b\u9244\u91cf\u304c\u4e00\u5b9a\u306b\u7dad\u6301\u3055\u308c\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u30d8\u30e0\u304c\u7d30\u80de\u5185\u306e\u9244\u6052\u5e38\u6027\u7dad\u6301\u306e\u305f\u3081\u306e\u30b7\u30b0\u30ca\u30eb\u5206\u5b50\u3067\u3042\u308b\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u308b\u3002\u4eca\u5f8c\u3001\u3053\u306e\u3088\u3046\u306a\u30d8\u30e0\u306b\u3088\u308b\u5236\u5fa1\u7cfb\u306e\u7834\u7dbb\u3068\u8ca7\u8840\u3001\u809d\u786c\u5909\u3001\u795e\u7d4c\u5909\u6027\u75be\u60a3\u3068\u3044\u3063\u305f\u9244\u4ee3\u8b1d\u7570\u5e38\u306e\u69d8\u3005\u306a\u75c5\u614b\u3068\u306e\u95a2\u9023\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u3067\u3001\u65b0\u305f\u306a\u6cbb\u7642\u6cd5\u3084\u767a\u75c5\u4e88\u9632\u7b49\u306e\u6307\u91dd\u304c\u5f97\u3089\u308c\u308b\u3068\u671f\u5f85\u3067\u304d\u308b\u3002<\/p><p>&nbsp;<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/1-s2.0-S0162013417307286-fx1_lrg-1-e1600246902320.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1560 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2020\/09\/1-s2.0-S0162013417307286-fx1_lrg-1-e1600246902320.jpg\" alt=\"\" width=\"600\" height=\"132\" \/><\/a><\/p><p>&nbsp;<\/p><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003986119305624\">Role of conserved arginine in the heme distal site of HutZ from Vibrio cholerae in the heme degradation reaction, Takeshi Uchida, Nobuhiko Dojun, Kazuki Ota, Yukari Sekine, Yuina Nakamura, Sayaka Umetsu and Koichiro Ishimori , Arch Biochem Biophys., 677, 108165 (15 pages) (2019) (DOI: 10.1016\/j.abb.2019.108165)<\/a><\/h2><div>\u30b3\u30ec\u30e9\u83cc\u7531\u6765\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bHutZ\u306f\u30d8\u30e0\u304c\u7d50\u5408\u3059\u308b\u90e8\u4f4d<wbr \/>\u306bArg92\u304c\u5b58\u5728\u3057\uff0c<wbr \/>\u3053\u306e\u6b8b\u57fa\u306f\u3053\u306e\u30d5\u30a1\u30df\u30ea\u30fc\u3067\u307b\u307c\u5b8c\u5168\u306b\u4fdd\u5b58\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001<wbr \/>\u6d3b\u6027\u306b\u5fc5\u9808\u3067\u3042\u308b\u3068\u4e88\u60f3\u3055\u308c\u3066\u3044\u305f\u3002\u4eca\u56de\u3001<wbr \/>\u3053\u306e\u6b8b\u57fa\u3092\u758e\u6c34\u6027\u6b8b\u57fa\u3067\u3042\u308b\u30a2\u30e9\u30cb\u30f3\u3001\u30ed\u30a4\u30b7\u30f3\u3001<wbr \/>\u307e\u305f\u306f\u30a2\u30eb\u30ae\u30cb\u30f3\u3068\u540c\u3058\u9178\u6027\u6b8b\u57fa\u3067\u3042\u308b\u30d2\u30b9\u30c1\u30b8\u30f3\u3001<wbr \/>\u30ea\u30b8\u30f3\u306b\u305d\u308c\u305e\u308c\u7f6e\u63db\u3057\u3001\u305d\u306e\u6a5f\u80fd\u3092\u691c\u8a0e\u3057\u305f\u3002<\/div><div>\u30d8\u30e0\u304c\u5206\u89e3\u3059\u308b\u969b\u306b\u9178\u7d20\u5206\u5b50\u304c\u30d8\u30e0\u306b\u7d50\u5408\u3057\u3001Arg92\u3068\u76f8\u4e92\u4f5c<wbr \/>\u7528\u3059\u308b\u3053\u3068\u306f\u30e9\u30de\u30f3\u30b9\u30da\u30af\u30c8\u30eb\u304b\u3089\u793a\u3055\u308c\u305f\u304c\u3001<wbr \/>\u30d8\u30e0\u306e\u5206\u89e3\u306e\u4f4d\u7f6e\u7279\u7570\u6027\u306b\u306f\u307b\u3068\u3093\u3069\u8ca2\u732e\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u3063<wbr \/>\u305f\u3002\u4e00\u65b9\u3001\u3053\u306eArg92\u3068\u9178\u7d20\u5206\u5b50\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u5f31\u307e\u308b\u3068\u4e8c\u91cf<wbr \/>\u4f53\u754c\u9762\u306e\u69cb\u9020\u304c\u5909\u5316\u3057\u3001\u9175\u7d20\u6d3b\u6027\u304c\u6e1b\u5c11\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002Ar<wbr \/>g92\u3068\u9178\u7d20\u5206\u5b50\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306f\u754c\u9762\u3092\u901a\u3058\u3001<wbr \/>\u30d8\u30e0\u306e\u53cd\u5bfe\u5074\u306b\u914d\u4f4d\u5b50\u3067\u3042\u308b\u30d2\u30b9\u30c1\u30b8\u30f3\u3068\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178\u306e\u6c34\u7d20\u7d50<wbr \/>\u5408\u3092\u5f31\u3081\u3001<wbr \/>\u7d50\u679c\u7684\u306b\u9175\u7d20\u6d3b\u6027\u304c\u6e1b\u5c11\u3059\u308b\u3068\u3044\u3046\u30d8\u30e0\u306e\u9060\u4f4d\u5074\u304b\u3089\u8fd1\u4f4d\u5074\u306b\u304b\u3051<wbr \/>\u3066\u306e\u69cb\u9020\u3092\u5909\u5316\u3092\u901a\u3058\u3001\u9175\u7d20\u6d3b\u6027\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u3057\u304f\u307f\uff08\u56f3\u53c2\u7167\uff09<wbr \/>\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3002<\/div><div><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/11\/ABB-Arg92.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1480 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/11\/ABB-Arg92-150x150.png\" alt=\"\" width=\"150\" height=\"150\" \/><\/a><\/div><h2><a href=\"https:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2019\/dt\/c9dt00926d#!divAbstract\">Role of His63 in HutZ from Vibrio cholerae in the heme degradation reaction and heme binding, Takeshi Uchida, Nobuhiko Dojun, Yukari Sekine and Koichiro Ishimori, Dalton Trans., in press<\/a><\/h2><div>\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20 (HO) \u306f\u307b\u307c100%\u306e\u5272\u5408\u3067\u3001\u03b1\u4f4d\u3067\u958b\u88c2\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001<wbr \/>\u30b3\u30ec\u30e9\u83cc\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bHutZ\u306f\u30d8\u30e0\u306e\u03b2\u4f4d\u3068\u03b4\u4f4d\u3092\u307b\u307c<wbr \/>1:1\u3067\u958b\u88c2\u3059\u308b\u3002<wbr \/>HO\u3067\u306f\u30d8\u30e0\u306e\u30d7\u30ed\u30d4\u30aa\u30f3\u9178\u3068\u6c34\u7d20\u7d50\u5408\u3059\u308b\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u304c\u5b58\u5728\u3057<wbr \/>\u3001\u3053\u306e\u6b8b\u57fa\u3092\u7f6e\u63db\u3059\u308b\u3068\u03b1\u4f4d\u4ee5\u5916\u3067\u3082\u958b\u88c2\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u308b\u3002<wbr \/>HutZ\u306b\u304a\u3044\u3066\u30d8\u30e0\u306e\u30d7\u30ed\u30d4\u30aa\u30f3\u9178\u8fd1\u508d\u306b\u552f\u4e00\u5b58\u5728\u3059\u308bHis6<wbr \/>3\u304c\u30d8\u30e0\u306e\u958b\u88c2\u90e8\u4f4d\u3092\u6c7a\u5b9a\u3057\u3066\u3044\u308b\u306e\u304b\u691c\u8a0e\u3057\u305f\u3002<br \/>\u305d\u306e\u7d50\u679c\u3001<wbr \/>His63\u3092\u7f6e\u63db\u3057\u3066\u3082\u30d8\u30e0\u306e\u958b\u88c2\u90e8\u4f4d\u306f\u5929\u7136\u578b\u3068\u5909\u308f\u3089\u306a\u304b\u3063\u305f<wbr \/>\u3053\u3068\u304b\u3089\u3001<wbr \/>HutZ\u306e\u30d8\u30e0\u306e\u958b\u88c2\u306f\u7279\u5b9a\u306e\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306b\u3088\u308a\u6c7a\u5b9a\u3059\u308b\u306e\u3067\u306f<wbr \/>\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u3053\u306e\u5909\u7570\u4f53\u306f\u30d8\u30e0\u306e\u89aa\u548c\u6027\u304c\u4f4e\u4e0b\u3057\u3001<wbr \/>\u30d8\u30e0\u306e\u5206\u89e3\u6d3b\u6027\u3082\u6d88\u5931\u3057\u305f\u3002FRET\u306b\u3088\u308b\u8ddd\u96e2\u5909\u5316\u306e\u89b3\u6e2c\u304b\u3089\u3001<wbr \/>\u4e8c\u91cf\u4f53\u754c\u9762\u306e\u611f\u899a\u304c\u5e83\u304c\u308a\u3001<wbr \/>\u4e8c\u91cf\u4f53\u9593\u306e\u76f8\u4e92\u4f5c\u7528\u304c\u5909\u5316\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002<wbr \/>\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3068\u7570\u306a\u308a\u4e8c\u91cf\u4f53\u3067\u3042\u308bHutZ\u306f\u305d\u306e\u4e8c\u91cf\u4f53\u306e<wbr \/>\u76f8\u5bfe\u914d\u7f6e\u3092\u5fae\u5c0f\u306b\u52d5\u304b\u3059\u3053\u3068\u3067\u3001<wbr \/>\u4e0d\u6d3b\u6027\u578b\u304b\u3089\u6d3b\u6027\u578b\u306b\u5909\u63db\u3059\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u3066\u304d\u305f\u304c\u3001<wbr \/>His63\u306f\u3053\u306e\u76f8\u5bfe\u914d\u7f6e\u306e\u5909\u5316\u306b\u95a2\u4e0e\u3057\u3066\u304a\u308a\u3001<wbr \/>\u30d8\u30e0\u5206\u89e3\u53cd\u5fdc\u306e\u9375\u3068\u306a\u308b\u30a2\u30df\u30ce\u9178\u3067\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002<\/div><div><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/03\/GA-H63L.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1437 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/03\/GA-H63L-150x150.png\" alt=\"\" width=\"150\" height=\"150\" \/><\/a><\/div><h2><a href=\"https:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2019\/dt\/c9dt00604d#!divAbstract\">Subunit-subunit interactions play a key role in the heme-degradation reaction of HutZ from Vibrio cholerae,\u00a0Takeshi Uchida, \u00a0Kazuki Ota, \u00a0Yukari Sekine, \u00a0Nobuhiko Dojun \u00a0and \u00a0Koichiro Ishimori, Dalton Trans., 2019,\u00a0<strong>48<\/strong>, 3973 <\/a><\/h2><p>HutZ\u306e\u30d8\u30e0\u5206\u89e3\u6d3b\u6027\u306fpH\u4f9d\u5b58\u7684\u3067\u3001pH 6.0\u3067\u6700\u5927\u3067\u3042\u308a\u3001pH\u3092\u9ad8\u304f\u3059\u308b\u306b\u3064\u308c\u3001\u6d3b\u6027\u306f\u6e1b\u5c11\u3057\u3001pH 8.0\u4ee5\u4e0a\u3067\u306f10%\u4ee5\u4e0b\u306e\u6d3b\u6027\u3057\u304b\u306a\u3044\u3002\u4eca\u56de\u3001<wbr \/>\u30d8\u30e0\u3068\u30d8\u30e0\u304b\u308920 \u00c5\u7a0b\u5ea6\u96e2\u308c\u305f\u4f4d\u7f6e\u306b\u5b58\u5728\u3059\u308bTrp109\u3068\u306e\u8ddd\u96e2\u5909\u5316\u3092FRET (F\u00f6rster\u00a0resonance energy transfer)\u3092\u7528\u3044\u3066\u89e3\u6790\u3057\u305f\u7d50\u679c\u3001pH 6\u3067\u306fpH 8\u306b\u6bd4\u3079\u3001\u7d041 \u00c5\u9577\u304f\u306a\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u4e8c\u91cf\u4f53\u754c\u9762\u306b\u5b58\u5728\u3059\u308bAla31\u3092Val\u306b\u7f6e\u63db\u3059\u308b\u3068\u3001<wbr \/>\u3053\u306e\u8ddd\u96e2\u304c7-9 \u00c5\u8fd1\u304f\u5897\u5927\u3057\u3001\u4e8c\u91cf\u4f53\u306e\u69cb\u9020\u304c\u5d29\u308c\u3001\u6d3b\u6027\u3092\u5931\u3046\u3002<wbr \/>\u4ee5\u4e0a\u306e\u3053\u3068\u304b\u3089\u3001<wbr \/>\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3068\u7570\u306a\u308a\u4e8c\u91cf\u4f53\u3067\u3042\u308bHutZ\u306f\u305d\u306e\u4e8c\u91cf\u4f53\u306e<wbr \/>\u76f8\u5bfe\u914d\u7f6e\u3092\u5fae\u5c0f\u306b\u52d5\u304b\u3059\u3053\u3068\u3067\u3001<wbr \/>\u4e0d\u6d3b\u6027\u578b\u304b\u3089\u6d3b\u6027\u578b\u306b\u5909\u63db\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/02\/Fig10-New-2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-thumbnail wp-image-1418 aligncenter colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2019\/02\/Fig10-New-2-150x150.png\" alt=\"\" width=\"150\" height=\"150\" \/><\/a><\/p><p>&nbsp;<\/p><h2><a href=\"http:\/\/www.jbc.org\/content\/early\/2018\/08\/09\/jbc.RA118.003579\">Structural insight into proline cis\/trans isomerization of unfolded proteins catalyzed by the Trigger Factor chaperone<\/a><\/h2><p dir=\"ltr\">\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u304a\u3044\u3066\u306f\uff0c\u30b8\u30b9\u30eb\u30d5\u30a3\u30c9\u7d50\u5408\u3084cis\u578b\u30d7\u30ed\u30ea\u30f3\u306a\u3069\u306e\u7279\u5b9a\u306e\u90e8\u5206\u69cb\u9020\u306e\u5f62\u6210\u304c\u91cd\u8981\u306b\u306a\u308b\u5834\u5408\u304c\u591a\u3044\u304c\uff0c\u3044\u304f\u3064\u304b\u306e\u5206\u5b50\u30b7\u30e3\u30da\u30ed\u30f3\u306f\u3053\u306e\u3088\u3046\u306a\u90e8\u5206\u69cb\u9020\u306e\u5f62\u6210\u3092\u89e6\u5a92\u3059\u308b\u6a5f\u80fd\u30c9\u30e1\u30a4\u30f3\u3092\u6709\u3057\u3066\u3044\u308b\uff0e\u30ea\u30dc\u30bd\u30fc\u30e0\u8fd1\u508d\u3067\u5909\u6027\u72b6\u614b\u306e\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308bTrigger Factor (TF) \u30b7\u30e3\u30da\u30ed\u30f3\u306fpeptidyl-prolyl cis\/trans isomerase domain (TFPPD) \u3092\u6301\u3061\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u5f8b\u901f\u6bb5\u968e\u3068\u3082\u306a\u308a\u3046\u308b\u30d7\u30ed\u30ea\u30f3\u306ecis\/trans\u7570\u6027\u5316\u53cd\u5fdc\u3092\u89e6\u5a92\u3059\u308b\uff0e\u3053\u306eTFPPD\u306e\u4f5c\u7528\u6a5f\u5e8f\u3092\u89e3\u660e\u3059\u308b\u305f\u3081\uff0c\u6211\u3005\u306f\u307e\u305a\uff0c\u5909\u6027\u72b6\u614b\u306e\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30d7\u30ed\u30ea\u30f3\u6b8b\u57fa\u3092\u542b\u3080\u9818\u57df\u3068TFPPD\u3068\u306e\u8907\u5408\u4f53\u7acb\u4f53\u69cb\u9020\u3092NMR\u6cd5\u306b\u3088\u308a\u6c7a\u5b9a\u3057\u305f\uff0e\u305d\u306e\u7d50\u679c\uff0c\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u758e\u6c34\u6027\u9818\u57df\u306b\u4f4d\u7f6e\u3059\u308b\u30d7\u30ed\u30ea\u30f3\u3068\u82b3\u9999\u65cf\u30a2\u30df\u30ce\u9178\u304cTFPPD\u306e\u758e\u6c34\u6027\u90e8\u4f4d\u306b\u3088\u3063\u3066\u8a8d\u8b58\u3055\u308c\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u3053\u306e\u30d7\u30ed\u30ea\u30f3-\u82b3\u9999\u65cf\u30a2\u30df\u30ce\u9178\u306e\u914d\u5217\u306f\uff0cTF\u304c\u8a8d\u8b58\u3059\u308b\u30d7\u30ed\u30ea\u30f3\u306b\u5171\u901a\u3057\u3066\u3044\u308b\u3053\u3068\u304b\u3089\uff0cTFPPD\u306f\u30d7\u30ed\u30ea\u30f3-\u82b3\u9999\u65cf\u30a2\u30df\u30ce\u9178\u3092\u7d50\u5408\u30e2\u30c1\u30fc\u30d5\u3068\u3059\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u6b21\u306b\uff0c\u5206\u5b50\u52d5\u529b\u5b66\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u305f\u3068\u3053\u308d\uff0ccis\/trans\u9077\u79fb\u306e\u9014\u4e2d\u6bb5\u968e\u306b\u304a\u3044\u3066TF Ile195\u3068\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u9593\u306b\u904e\u6e21\u7684\u306a\u5206\u5b50\u9593\u6c34\u7d20\u7d50\u5408\u304c\u5f62\u6210\u3055\u308c\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u3055\u3089\u306b\uff0c\u5909\u7570\u4f53\u3092\u7528\u3044\u305f\u6d3b\u6027\u6e2c\u5b9a\u3084NMR\u7de9\u548c\u89e3\u6790\u306b\u3088\u3063\u3066\uff0c\u3053\u306e\u5206\u5b50\u9593\u6c34\u7d20\u7d50\u5408\u304cTF\u306e\u89e6\u5a92\u6d3b\u6027\u306b\u5fc5\u9808\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u305f\uff0e\u4ee5\u4e0a\u306e\u3088\u3046\u306bTF\u306f\uff0c\u30d7\u30ed\u30ea\u30f3-\u82b3\u9999\u65cf\u30e2\u30c1\u30fc\u30d5\u4e2d\u306e\u30d7\u30ed\u30ea\u30f3\u306b\u5bfe\u3057\u3066\u4f5c\u7528\u3057\uff0c\u5206\u5b50\u9593\u6c34\u7d20\u7d50\u5408\u306b\u3088\u308b\u9077\u79fb\u72b6\u614b\u306e\u5b89\u5b9a\u5316\u306b\u3088\u3063\u3066cis\/trans\u7570\u6027\u5316\u3092\u4fc3\u9032\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\uff0c\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c40\u6240\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u4fc3\u9032\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u308b\uff0e<\/p><p dir=\"ltr\"><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/Graphical-abstract.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1386 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/Graphical-abstract-150x150.png\" alt=\"Graphical-abstract\" width=\"150\" height=\"150\" \/><\/a><\/p><h2><a href=\"https:\/\/elifesciences.org\/articles\/35731\"><span style=\"font-size: medium;\">Oligomerization of a molecular chaperone modulates its activity, Tomohide Saio, Soichiro Kawagoe, Koichiro Ishimori, Charalampos G Kalodimos, eLife, 35731(2018)\u00a0<\/span><\/a><\/h2><p>\u5206\u5b50\u30b7\u30e3\u30da\u30ed\u30f3\u306f\u7acb\u4f53\u69cb\u9020\u3092\u5f62\u6210\u3059\u308b\u524d\u306e\u65b0\u751f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u8a8d\u8b58\u3057\uff0c\u305d\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u5236\u5fa1\u3059\u308b\u304c\uff0c\u305d\u306e\u8a73\u7d30\u306a\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u660e\u3089\u304b\u306b\u306a\u3063\u3066\u3044\u306a\u3044\uff0e\u672c\u7814\u7a76\u3067\u306f\uff0c\u5927\u8178\u83cc\u7d30\u80de\u8cea\u3067\u6a5f\u80fd\u3059\u308b\u30c8\u30ea\u30ac\u30fc\u30d5\u30a1\u30af\u30bf\u30fc (TF) \u30b7\u30e3\u30da\u30ed\u30f3\u306b\u7740\u76ee\u3057\uff0cTF\u304c\u5358\u91cf\u4f53\u30fb\u30cb\u91cf\u4f53\u306e\u30cb\u72b6\u614b\u3092\u5207\u308a\u66ff\u3048\u306a\u304c\u3089\u591a\u5f69\u306a\u6a5f\u80fd\u3092\u767a\u63ee\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\uff0e100 kDa \u30cb\u91cf\u4f53TF\u306e\u9ad8\u5206\u89e3\u80fd\u7acb\u4f53\u69cb\u9020\u3092NMR\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3057\u305f\u7d50\u679c\uff0cTF\u4e0a\u306b\u3042\u308b\u8907\u6570\u306e\u57fa\u8cea\u7d50\u5408\u30b5\u30a4\u30c8\u306f\uff0c\u30cb\u91cf\u4f53\u5f62\u6210\u306b\u3088\u3063\u3066\u90e8\u5206\u7684\u306b\u8986\u3044\u96a0\u3055\u308c\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u3053\u308c\u306f\uff0c\u30cb\u91cf\u4f53TF\u306f\u5358\u91cf\u4f53\u3088\u308a\u3082\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u89aa\u548c\u6027\u304c\u5f31\u3044\u3068\u3044\u3046\u5b9f\u9a13\u7d50\u679c\u3068\u3082\u5408\u81f4\u3057\uff0c\u4e00\u898b\uff0cTF\u30cb\u91cf\u4f53\u304c\u4e0d\u6d3b\u6027\u578b\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3059\u304b\u306e\u3088\u3046\u306b\u601d\u308f\u308c\u305f\uff0e\u3057\u304b\u3057\uff0c\u86cd\u5149\u30b9\u30c8\u30c3\u30d7\u30c8\u30fb\u30d5\u30ed\u30fc\u306b\u3088\u308a\u57fa\u8cea\u3068\u306e\u7d50\u5408-\u89e3\u96e2\u901f\u5ea6\u3092\u8a08\u6e2c\u3057\u305f\u7d50\u679c\uff0cTF\u30cb\u91cf\u4f53\u306f\u5358\u91cf\u4f53\u3088\u308a\u3082\u57fa\u8cea\u3068\u306e\u7d50\u5408\u901f\u5ea6\u304c\u901f\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u3055\u3089\u306b\uff0c\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6298\u308a\u305f\u305f\u307f\u306b\u304a\u3051\u308bTF\u306e\u5f71\u97ff\u3092\u8a55\u4fa1\u3057\u305f\u3068\u3053\u308d\uff0cTF\u30cb\u91cf\u4f53\u306f\u57fa\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5909\u6027\u72b6\u614b\u306b\u4fdd\u6301\u3059\u308b\u80fd\u529b\u304c\u5358\u91cf\u4f53\u3088\u308a\u3082\u9ad8\u3044\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0eTF\u4e8c\u91cf\u4f53\u306e\u7acb\u4f53\u69cb\u9020\u4e0a\u3067\u306f\u8907\u6570\u306e\u57fa\u8cea\u7d50\u5408\u30b5\u30a4\u30c8\u304c\u4e92\u3044\u306b\u8fd1\u63a5\u3057\uff0c\u4e00\u3064\u306e\u5927\u304d\u306a\u9762\u3092\u5f62\u6210\u3057\u3066\u3044\u308b\u304c\uff0c\u3053\u306e\u57fa\u8cea\u7d50\u5408\u30b5\u30a4\u30c8\u306e\u96c6\u7a4d\u304c\u7d50\u5408\u901f\u5ea6\u3092\u4e0a\u6607\u3055\u305b\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\uff0eTF\u306f\u4e8c\u91cf\u4f53\u5316\u306b\u3088\u3063\u3066\u901f\u3044\u7d50\u5408\u901f\u5ea6\u3092\u9054\u6210\u3057\uff0c\u3088\u308a\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u51dd\u96c6\u6291\u5236\u3068\u5909\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8f38\u9001\u3092\u53ef\u80fd\u306b\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\uff0e<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/image_v2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1361 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/image_v2-150x150.png\" alt=\"image_v2\" width=\"150\" height=\"150\" \/><\/a><\/p><p>&nbsp;<\/p><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0304416518300722\">Polyethylene glycol promotes autoxidation of cytochrome\u00a0c,\u00a0Wataru Sato, Takeshi Uchida, Tomohide Saio, and Koichiro Ishimori, Biochim. Biophys. Acta,\u00a02018, 1862, 1339-1349<\/a><\/h2><p>\u96fb\u5b50\u30ad\u30e3\u30ea\u30a2\u30bf\u30f3\u30d1\u30af\u8cea\u30b7\u30c8\u30af\u30ed\u30e0<i>c<\/i>(Cyt\u00a0<i>c<\/i>)\u306fATP\u5408\u6210\u306b\u5fc5\u9808\u306e\u904e\u7a0b\u306b\u76f8\u5f53\u3059\u308b\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u96fb\u5b50\u4f1d\u9054<wbr \/>\u7cfb\u306b\u304a\u3044\u3066\u3001\u8907\u5408\u4f53III\u304b\u3089\u30b7\u30c8\u30af\u30ed\u30e0<i>c<\/i>\u9178\u5316\u9175\u7d20(C<i>c<\/i>O)\u3078<wbr \/>\u306e\u96fb\u5b50\u79fb\u52d5\u53cd\u5fdc\u3092\u4ef2\u4ecb\u3059\u308b\u5f79\u5272\u3092\u62c5\u3046\u3002\u672c\u7814\u7a76\u3067\u306f\u3053\u306eCyt\u00a0<i>c<\/i>\u304c\u5408\u6210\u9ad8\u5206\u5b50\u30dd\u30ea\u30a8\u30c1\u30ec\u30f3\u30b0\u30ea\u30b3\u30fc\u30eb(PEG)\u5b58\u5728\u4e0b\u306b\u304a\u3044\u3066<wbr \/>\u30d8\u30e0\u306e\u9084\u5143\u96fb\u4f4d\u304c40 mV\u7a0b\u5ea6\u30c0\u30a6\u30f3\u30b7\u30d5\u30c8\u3057\u3001\u81ea\u52d5\u9178\u5316\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u3053\u306eCy<wbr \/>t\u00a0<i>c<\/i>\u306e\u81ea\u52d5\u9178\u5316\u306e\u8981\u56e0\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u7a2e\u3005\u306e\u5206\u5149\u6e2c\u5b9a\u3092\u884c\u3063\u305f\u7d50<wbr \/>\u679c\u3001PEG\u3092\u6eb6\u6db2\u4e2d\u306b\u52a0\u3048\u308b\u3053\u3068\u3067Cyt\u00a0<i>c<\/i>\u00a0\u8868\u9762\u304b\u3089\u8131\u6c34\u548c\u304c\u4fc3\u9032\u3055\u308c\u3001\u305d\u308c\u3092\u5f15\u304d\u91d1\u306b\u30d8\u30e0\u306e1 \u212b\u7a0b\u5ea6\u306e\u4f4d\u7f6e\u5909\u5316\u3092\u4f34\u3046\u5fae\u7d30\u306a\u69cb\u9020\u5909\u5316\u304c\u8a98\u8d77\u3055\u308c\u308b\u3053\u3068\u304c\u793a\u3055\u308c<wbr \/>\u305f\u3002\u3055\u3089\u306b\u5909\u7570\u4f53Cyt\u00a0<i>c<\/i>\u3092\u7528\u3044\u305f\u5b9f\u9a13\u3088\u308a\u3001\u81ea\u52d5\u9178\u5316\u306b\u81f3\u308b\u69cb\u9020\u5909\u5316\u306e\u539f\u56e0\u3068\u306a\u308b\u8131\u6c34\u548c<wbr \/>\u304c\u30d8\u30e0\u8fd1\u508d\u306b\u4f4d\u7f6e\u3059\u308b\u758e\u6c34\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57faIle81, Val83\u5468\u8fba\u304b\u3089\u306e\u3082\u306e\u3067\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u3053\u308c\u3089\u306e<wbr \/>\u758e\u6c34\u6027\u6b8b\u57fa\u306fCyt\u00a0<i>c<\/i>-C<i>c<\/i>O\u8907\u5408\u4f53\u306e\u30c9\u30c3\u30ad\u30f3\u30b0\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3(Sato et al., J. Biol. Chem., 2016)\u3088\u308aC<i>c<\/i>O\u3068\u306e\u4f1a\u5408\u6642\u306b\u8868\u9762\u304b\u3089\u8131\u6c34\u548c\u304c\u8a98\u8d77\u3055\u308c\u308b\u3053<wbr \/>\u3068\u304c\u4e88\u6e2c\u3055\u308c\u3066\u304a\u308a\u3001\u672c\u7814\u7a76\u3067\u793a\u3055\u308c\u305fCyt\u00a0<i>c<\/i>\u30d8\u30e0\u8fd1\u508d\u306e\u6c34\u548c\u74b0\u5883\u5909\u5316\u304c\u9084\u5143\u96fb\u4f4d\u306e\u4f4e\u4e0b\u3092\u901a\u3057\u3066\u9ad8\u52b9\u7387\u306aC<i>c<\/i><wbr \/>O\u3078\u306e\u96fb\u5b50\u4f1d\u9054\u53cd\u5fdc\u3092\u4fc3\u9032\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2018\/03\/BBA_abstract.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1317 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2018\/03\/BBA_abstract-150x150.png\" alt=\"BBA_abstract\" width=\"150\" height=\"150\" \/><\/a><\/p><p>&nbsp;<\/p><h2><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0162013417307286\">Redox-dependent axial ligand replacement and its functional significance\u00a0in heme-bound iron regulatory proteins, Mariko Ogura, Ryosuke Endo, Haruto Ishikawa, Yukiko Takeda, Takeshi\u00a0Uchida, Kazuhiro Iwai, Kazuo Kobayashi, Koichiro Ishimori,\u00a0Journol of Inorg. Biochem., 2018, 182, 232-248<\/a><\/h2><p dir=\"ltr\">\u9244\u5236\u5fa1\u86cb\u767d\u8ceaIron regulatory proteins (IRPs\uff09\u306f\u3001\u54fa\u4e73\u985e\u306e\u7d30\u80de\u5185\u9244\u6fc3\u5ea6\u306e\u8abf\u7bc0\u56e0\u5b50\u3067\u3042\u308a\u3001\u9244\u306e\u53d6\u308a\u8fbc\u307f\u3084\u8caf\u8535\u304a\u3088\u3073\u5229\u7528\u306b\u95a2\u4e0e\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7ffb\u8a33\u91cf\u3092\u5236\u5fa1\u3059\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u5185\u306e\u9244\u6fc3\u5ea6\u3092\u4e00\u5b9a\u306b\u4fdd\u3063\u3066\u3044\u308b\u3002IRPs\u306b\u306f\u30a2\u30df\u30ce\u9178\u914d\u5217\u306e\u3088\u304f\u4f3c\u305fIRP1\u3068IRP2\u304c\u5b58\u5728\u3057\u3001\u3069\u3061\u3089\u3082\u30d8\u30e0\u3092\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u90e8\u4f4d\u3068\u3057\u3066\u3067\u306f\u306a\u304f\u3001\u305d\u306e\u6a5f\u80fd\u3092\u5236\u5fa1\u3059\u308b\u5206\u5b50\u3068\u3057\u3066\u7d50\u5408\u3067\u304d\u308bheme regulatory motif (HRM)\u90e8\u4f4d\u3092\u3082\u3063\u3066\u3044\u308b\u3002HRM\u3078\u306e\u30d8\u30e0\u306e\u7d50\u5408\u306f\u3053\u308c\u307e\u3067IRP2\u306b\u3064\u3044\u3066\u306e\u307f\u5831\u544a\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u306eHRM\u306b\u7d50\u5408\u3057\u305f\u30d8\u30e0\u306f\u3001\u9084\u5143\u5264\u306e\u5b58\u5728\u4e0b\u3001\u9178\u7d20\u5206\u5b50\u3068\u53cd\u5fdc\u3059\u308b\u3053\u3068\u3067\u6d3b\u6027\u9178\u7d20\u7a2e\uff08ROS\uff09\u3092\u751f\u6210\u3057\u3001\u305d\u306eROS\u304c\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306e\u9178\u5316\u53cd\u5fdc\u3092\u5f15\u304d\u8d77\u3053\u3059\u3053\u3068\u3067\u3001\u7d30\u80de\u5185\u9244\u6fc3\u5ea6\u8abf\u7bc0\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u767a\u4fe1\u3055\u308c\u308b\u3068\u60f3\u5b9a\u3055\u308c\u3066\u3044\u308b\u3002\u3057\u304b\u3057\u3001\u3053\u306e\u3088\u3046\u306aROS\u306f\u53cd\u5fdc\u6027\u304c\u9ad8\u304f\u3001\u7d30\u80de\u5185\u3067\u751f\u6210\u3059\u308b\u3068\u5468\u56f2\u306e\u751f\u4f53\u7d44\u7e54\u3092\u640d\u50b7\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001IRP1\u306eHRM\u306b\u3082\u30d8\u30e0\u304c\u9078\u629e\u7684\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u3001\u305d\u306e\u30d8\u30e0\u914d\u4f4d\u74b0\u5883\u306f\u3001HRM\u3092\u30d8\u30e0\u7d50\u5408\u90e8\u4f4d\u306b\u6301\u3064IRP2\u3068\u975e\u5e38\u306b\u985e\u4f3c\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3002\u3057\u304b\u3057\u3001IRP1\u306eHRM\u3078\u306e\u30d8\u30e0\u306e\u7d50\u5408\u306fROS\u306e\u751f\u6210\u3084\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306e\u9178\u5316\u4fee\u98fe\u3092\u5f15\u304d\u8d77\u3053\u3055\u305a\u3001\u3053\u308c\u306f\u30d8\u30e0\u306b\u3088\u308bROS\u751f\u6210\u53cd\u5fdc\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u53cd\u5fdc\u4e2d\u9593\u4f53\u304c\u3001IRP1\u3067\u306f\u5f62\u6210\u3055\u308c\u306a\u3044\u305f\u3081\u3067\u3042\u308b\u3053\u3068\u3092\u7a81\u304d\u6b62\u3081\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u672c\u8ad6\u6587\u3067\u306f\u3001\u7d30\u80de\u6bd2\u3068\u306a\u308a\u3046\u308bROS\u3092\u751f\u6210\u305b\u305a\u306b\u30d8\u30e0\u3092\u7d30\u80de\u5185\u9244\u6fc3\u5ea6\u306e\u30b7\u30b0\u30ca\u30eb\u5206\u5b50\u3068\u3057\u3066\u7528\u3044\u308b\u65b0\u305f\u306a\u7d30\u80de\u5185\u9244\u6fc3\u5ea6\u306e\u5236\u5fa1\u6a5f\u69cb\u3092\u63d0\u5531\u3057\u305f\u3002<\/p><p>&nbsp;<\/p><h2><a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acschembio.7b00934\"><strong><span style=\"font-size: 15.99px;\">Heme Binding to Porphobilinogen Deaminase from Vibrio cholerae Decelerates the Formation of 1-Hydroxymethylbilane<\/span>,\u00a0Takeshi Uchida, Takumi Funamizu, Minghao Chen, Yoshikazu Tanaka,and Koichiro Ishimori, ACS Chem.\u00a0Biol.,2018, 13(3), 750-760<\/strong><\/a><\/h2><div>\u30dd\u30eb\u30d5\u30a9\u30d3\u30ea\u30ce\u30fc\u30b2\u30f3\u30c7\u30a2\u30df\u30ca\u30fc\u30bc (PBGD) \u306f\u3001<wbr \/>\u30dd\u30eb\u30d5\u30a9\u30d3\u30ea\u30ce\u30fc\u30b2\u30f3\u304b\u3089\u30d2\u30c9\u30ed\u30ad\u30b7\u30e1\u30c1\u30eb\u30d3\u30e9\u30f3\u3092\u5408\u6210\u3059\u308b\u9175\u7d20<wbr \/>\u3067\u3001\u30d8\u30e0\u5408\u6210\u306e3\u6bb5\u968e\u76ee\uff08\u307e\u305f\u306f4\u6bb5\u968e\u76ee\uff09\u306e\u53cd\u5fdc\u3092\u89e6\u5a92\u3059\u308b\u3002<wbr \/>\u3053\u306e\u6bb5\u968e\u306e\u751f\u6210\u7269\u304b\u3089\u74b0\u72b6\u306b\u8fd1\u3044\u69cb\u9020\u306b\u306a\u308b\u3053\u3068\u304b\u3089\u3001<wbr \/>\u30d8\u30e0\u306b\u3088\u308b\u9175\u7d20\u53cd\u5fdc\u306e\u963b\u5bb3\u306e\u53ef\u80fd\u6027\u306b\u3064\u3044\u3066\u6ce8\u76ee\u3057\u305f\u3002<wbr \/>PBGD\u306b\u30d8\u30e0\u304c\u7d50\u5408\u3059\u308b\u304b\u8abf\u3079\u305f\u3068\u3053\u308d\u3001<wbr \/>Cys105\u306b\u30d8\u30e0\u304c\u7d50\u5408\u3057\u3001\u305d\u306e\u7d50\u679c\u3001<wbr \/>PBGD\u306e\u9175\u7d20\u6d3b\u6027\u306f\u7d0420%\u6e1b\u5c11\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u305f\u3060\u3057\u3001<wbr \/>Cys105\u306b\u30d8\u30e0\u304c\u7d50\u5408\u3059\u308b\u3060\u3051\u3067\u306f\u6d3b\u6027\u306b\u5909\u5316\u306f\u306a\u304f\u3001<wbr \/>Cys105\u306e\u8fd1\u304f\u306b\u5b58\u5728\u3059\u308bHis227\u304c\u3055\u3089\u306b\u30d8\u30e0\u306b\u914d\u4f4d\u3059<wbr \/>\u308b\u3053\u3068\u3067\u3001\u30c9\u30e1\u30a4\u30f3\u9593\u306e\u76f8\u5bfe\u914d\u7f6e\u304c\u5909\u5316\u3057\u3001<wbr \/>\u6d3b\u6027\u304c\u6e1b\u5c11\u3059\u308b\u3053\u3068\u304c\u5909\u7570\u4f53\u3084\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u3088\u308a\u793a\u3055\u308c\u305f\u3002<wbr \/>\u30d8\u30e0\u306f\u751f\u4f53\u306b\u5fc5\u9808\u306e\u5206\u5b50\u3067\u3042\u308b\u4e00\u65b9\u3001<wbr \/>\u904e\u5270\u306b\u5b58\u5728\u3059\u308b\u3068\u7d30\u80de\u6bd2\u306b\u3082\u306a\u308b\u3053\u3068\u304b\u3089\u3001<wbr \/>\u904e\u5270\u306b\u30d8\u30e0\u304c\u5b58\u5728\u3059\u308b\u3068\u30d8\u30e0\u5408\u6210\u306b\u95a2\u4fc2\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7d50\u5408\u3057\u3001<wbr \/>\u305d\u306e\u5408\u6210\u3092\u6291\u5236\u3059\u308b\u3088\u3046\u306a\u8ca0\u306e\u30d5\u30a3\u30fc\u30c9\u30d0\u30c3\u30af\u6a5f\u69cb\u304c\u5b58\u5728\u3059\u308b\u3053\u3068<wbr \/>\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f<\/div><p>&nbsp;<\/p><h2><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.biochem.7b00152\">Heme Proximal Hydrogen Bonding between His170 and Asp132 Plays an Essential Role in the Heme Degradation Reaction of HutZ from Vibrio cholerae,\u00a0Takeshi Uchida, Nobuhiko Dojun, Yukari Sekine, and Koichiro Ishimori, Biochemistry, 2017, 56 (21), 2723\u20132734.<\/a><\/h2><p>\u897f\u6d0b\u30ef\u30b5\u30d3\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc\uff08HRP\uff09<wbr \/>\u306e\u3088\u3046\u306a\u30d8\u30e0\u9178\u5316\u9175\u7d20\u3067\u306f\u30d8\u30e0\u306b\u914d\u4f4d\u3057\u305f\u30d2\u30b9\u30c1\u30b8\u30f3\u304c\u30a2\u30b9\u30d1\u30e9\u30ae<wbr \/>\u30f3\u9178\u3084\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3068\u6c34\u7d20\u7d50\u5408\u3092\u5f62\u6210\u3057\u3001\u30a4\u30aa\u30f3\u5316\u3059\u308b\u3053\u3068\u3067\u3001<wbr \/>\u9ad8\u3044\u9178\u7d20\u6d3b\u6027\u80fd\u3092\u767a\u63ee\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002<wbr \/>\u6211\u3005\u304c\u767a\u898b\u3057\u305f\u30b3\u30ec\u30e9\u83cc\u7531\u6765\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bHutZ\u306b\u306f\u30d8<wbr \/>\u30e0\u5206\u89e3\u9175\u7d20\u3068\u3057\u3066\u306f\u4f8b\u5916\u7684\u306b\u3053\u306e\u6c34\u7d20\u7d50\u5408\u304c\u5b58\u5728\u3057\u3066\u304a\u308a\u3001<wbr \/>\u3053\u308c\u306b\u3088\u308aHutZ\u306e\u30d8\u30e0\u5206\u89e3\u6d3b\u6027\u304c\u4e2d\u6027\u3067\u6291\u5236\u3055\u308c\u3066\u3044\u308b\u3068\u4e88\u60f3<wbr \/>\u3055\u308c\u305f\u3002\u305d\u3053\u3067\u3001<wbr \/>\u30d8\u30e0\u5206\u89e3\u6d3b\u6027\u306b\u304a\u3051\u308b\u3053\u306e\u6c34\u7d20\u7d50\u5408\u306e\u5f79\u5272\u3092\u77e5\u308b\u305f\u3081\u3001<wbr \/>\u30d2\u30b9\u30c1\u30b8\u30f3\u304a\u3088\u3073\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178\u306e\u5909\u7570\u4f53\u3092\u4f5c\u6210\u3057\u3001<wbr \/>\u305d\u308c\u3089\u306e\u9175\u7d20\u6d3b\u6027\u3092\u8abf\u3079\u305f\u3002\u305d\u306e\u7d50\u679c\u3001<wbr \/>\u3053\u306e\u6c34\u7d20\u7d50\u5408\u3092\u5207\u65ad\u3059\u308b\u3068\u30d8\u30e0\u5206\u89e3\u6d3b\u6027\u306f\u6d88\u5931\u3057\u3001<wbr \/>\u30d8\u30e0\u306e\u7d50\u5408\u80fd\u3082\u6e1b\u5c11\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001<wbr \/>HutZ\u306f\u30d2\u30b9\u30c1\u30b8\u30f3\u3068\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u9178\u304c\u5f62\u6210\u3059\u308b\u6c34\u7d20\u7d50\u5408\u3092\u5207\u65ad<wbr \/>\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u305d\u306e\u5f37\u5ea6\u3092\u5fae\u8abf\u6574\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u3001<wbr \/>\u9175\u7d20\u6d3b\u6027\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-1216 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-300x155.png\" alt=\"TOC-Asp132\" width=\"300\" height=\"155\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-300x155.png 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-1024x532.png 1024w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-690x358.png 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-540x280.png 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-340x176.png 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-223x115.png 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-Asp132-124x64.png 124w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p><h2><a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2017\/dt\/c7dt01562c#!divAbstract\">Reaction Intermediates in the Heme Degradation Rea<strong>ction by HutZ f<\/strong>rom Vibrio cholerae,\u00a0Takeshi Uchida,\u00a0\u00a0Yukari Sekine,\u00a0\u00a0Nobuhiko Dojun,\u00a0\u00a0Ariel Lewis,\u00a0\u00a0Izumi Ishigami,\u00a0\u00a0Toshitaka Matsui,\u00a0\u00a0Syun-Ru Yeh\u00a0 and \u00a0Koichiro Ishimori, Dalton Trans., 2017, <strong>46<\/strong>, 8104<\/a><\/h2><p>\u6211\u3005\u306f\u3059\u3067\u306b\u3001<wbr \/>\u4ee3\u8868\u7684\u306a\u75c5\u539f\u83cc\u306e\u4e00\u3064\u3067\u3042\u308bHuZ\u304c\u30d8\u30e0\u306e\u5206\u89e3\u9175\u7d20\u3067\u3042\u308b\u3053\u3068\u306f<wbr \/>\u3059\u3067\u306b\u660e\u3089\u304b\u306b\u3057\u3066\u304d\u305f\u304c\u3001\u4eca\u56de\u3001<wbr \/>\u4e0d\u5b89\u5b9a\u3067\u89b3\u6e2c\u304c\u56f0\u96e3\u3067\u3042\u3063\u305f\u9178\u7d20\u4ed8\u52a0\u4f53\u3068\u30e1\u30bd-<wbr \/>\u30d2\u30c9\u30ed\u30ad\u30b7\u30d8\u30e0\u306e\u4e8c\u3064\u306e\u4e2d\u9593\u4f53\u3092\u89b3\u6e2c\u3057\u3001<wbr \/>\u53cd\u5fdc\u6a5f\u69cb\u306e\u6c7a\u5b9a\u306b\u6210\u529f\u3057\u305f\u3002\u9178\u7d20\u4ed8\u52a0\u4f53\u306f\u3001<wbr \/>\u9084\u5143\u72b6\u614b\u306eHutZ\u3068\u9178\u7d20\u5206\u5b50\u3092\u9ad8\u901f\u6df7\u5408\u88c5\u7f6e\u3092\u7528\u3044\u6df7\u5408\u3057\u3001<wbr \/>\u6df7\u5408\u5f8c\u300110~350 \u30df\u30ea\u79d2\u5f8c\u306e\u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u3068\u5171\u9cf4\u30e9\u30de\u30f3\u30b9\u30da\u30af\u30c8\u30eb\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068<wbr \/>\u306b\u3088\u308a\u540c\u5b9a\u3057\u305f\u3002\u307e\u305f\u3001\u30e1\u30bd-\u30d2\u30c9\u30ed\u30ad\u30b7\u30d8\u30e0\u306f\u3001<wbr \/>\u9178\u7d20\u5206\u5b50\u5b58\u5728\u3059\u308b\u3068\u305f\u3060\u3061\u306b\u5b89\u5b9a\u306a\u30d9\u30eb\u30c9\u30d8\u30e0\u306b\u5909\u63db\u3055\u308c\u308b\u3053\u3068\u304b<wbr \/>\u3089\u3001\u9178\u7d20\u6fc3\u5ea6\u304c1 ppm\u4ee5\u4e0b\u306e\u74b0\u5883\u4e0b\u3067\u7b49\u91cf\u306e\u904e\u9178\u5316\u6c34\u7d20\u3068\u53cd\u5fdc\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u89b3<wbr \/>\u6e2c\u306b\u6210\u529f\u3057\u305f\u3002\u3055\u3089\u306b\u3001<wbr \/>HutZ\u304c\u751f\u6210\u3059\u308b\u30d9\u30eb\u30c9\u30d8\u30e0\u306f\u4e2d\u5fc3\u91d1\u5c5e\u306e\u9244\u304c3\u4fa1\u3067\u3042\u308b\u305f\u3081\uff08<wbr \/>\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u306f2\u4fa1\uff09\u3001<wbr \/>\u9178\u5316\u6d3b\u6027\u3092\u6709\u3059\u308b\u3068\u3044\u3046\u7279\u5fb4\u3082\u3064\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u672c\u7814\u7a76\u306b\u3088\u308a\u660e\u3089\u304b\u306b\u3055\u308c\u305f\u75c5\u539f\u83cc\u306e\u30d8\u30e0\u5206\u89e3\u6a5f\u69cb\u306f\u3001<wbr \/>\u65b0\u898f\u306a\u6297\u83cc\u5264\u306e\u958b\u767a\u3078\u306e\u6709\u52b9\u306a\u77e5\u898b\u3068\u306a\u308b\u3053\u3068\u304c\u671f\u5f85\u3055\u308c\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-1187 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-300x224.png\" alt=\"TOC-DT\" width=\"300\" height=\"224\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-300x224.png 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-1024x765.png 1024w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-690x515.png 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-540x403.png 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-340x254.png 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-223x166.png 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-DT-124x92.png 124w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p><h2><a href=\"http:\/\/www.journal.csj.jp\/doi\/abs\/10.1246\/bcsj.20170104\">Heme Iron Coordination Structure of Heme\u00a0Transport Protein HutB from\u00a0<em>Vibrio cholerae<\/em>,<em>\u00a0<\/em>Takeshi Uchida, Takumi Funamizu, Mariko Ogura, and Koichiro Ishimori, Bull. Chem. Soc. Jpn., 2017, 90, 8, 924-930<\/a><\/h2><p>HutB\u306f\u907a\u4f1d\u5b50\u914d\u5217\u304b\u3089\u30b3\u30ec\u30e9\u83cc\u306e\u5916\u819c\u3068\u5185\u819c\u306b\u631f\u307e\u308c\u305f\u9818\u57df\u306b<wbr \/>\u5b58\u5728\u3059\u308b\u30d8\u30e0\u8f38\u9001\u3068\u4e88\u60f3\u3055\u308c\u3066\u3044\u305f\u3002<wbr \/>\u6211\u3005\u306f\u3053\u306eHutB\u306e\u767a\u73fe\u7cfb\u3092\u69cb\u7bc9\u3057\u3001<wbr \/>\u30d8\u30e0\u306e\u914d\u4f4d\u80fd\u3068\u914d\u4f4d\u69cb\u9020\u306b\u3064\u3044\u3066\u8abf\u3079\u305f\u3002<wbr \/>N\u672b\u7aef\u306e22\u6b8b\u57fa\u3092\u542b\u3080\u5168\u9577\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u53ef\u6eb6\u6027\u306e\u51dd\u96c6\u4f53\u3092\u5f62\u6210\u3057<wbr \/>\u305f\u304c\u3001\u3053\u306e\u90e8\u4f4d\u3092\u524a\u9664\u3059\u308b\u3068\u5358\u91cf\u4f53\u3068\u306a\u3063\u305f\u3053\u3068\u304b\u3089\u3001<wbr \/>N\u672b\u7aef\u306e22\u6b8b\u57fa\u306f\u30b7\u30b0\u30ca\u30eb\u914d\u5217\u3067\u3042\u308a\u3001\u819c\u9593\u90e8\u3078\u79fb\u52d5\u3059\u308b\u969b\u3001<wbr \/>\u3053\u306e\u90e8\u4f4d\u304c\u5207\u65ad\u3055\u308c\u3001\u5358\u91cf\u4f53\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u3055\u3089\u306b\u3001\u30a2\u30df\u30ce\u9178\u7f6e\u63db\u4f53\u304b\u3089\u3001<wbr \/>Tyr65\u3068Tyr198\u304c\u30d8\u30e0\u306b\u914d\u4f4d\u3057\u3001<wbr \/>His164\u304c\u305d\u306e\u30d8\u30e0\u306e\u914d\u4f4d\u3092\u88dc\u52a9\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u3053\u306e\u3088\u3046\u306a\u30d8\u30e0\u306e\u914d\u4f4d\u69cb\u9020\u306f\u3001<wbr \/>\u6211\u3005\u304c\u3059\u3067\u306b\u660e\u3089\u304b\u306b\u3057\u305f\u7d30\u80de\u8cea\u5185\u90e8\u3067\u306e\u30d8\u30e0\u306e\u8f38\u9001\u30bf\u30f3\u30d1\u30af\u8cea\uff08<wbr \/>HutX\uff09\u3068\u540c\u3058\u3067\u3042\u308a\u3001<wbr \/>\u30b3\u30ec\u30e9\u83cc\u306e\u30d8\u30e0\u8f38\u9001\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5171\u901a\u306e\u6027\u8cea\u3092\u898b\u51fa\u3057\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-1186 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-269x300.png\" alt=\"TOC-HutB-HP\" width=\"269\" height=\"300\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-269x300.png 269w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-690x768.png 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-540x601.png 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-340x378.png 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-223x248.png 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP-124x138.png 124w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-HutB-HP.png 720w\" sizes=\"(max-width: 269px) 100vw, 269px\" \/><\/a><\/p><h2>\u00a0<a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.biochem.6b01304\">The iron chaperone protein CyaY from <em>Vibrio cholerae<\/em> is a heme-binding protein, Takeshi Uchida, \u00a0Noriyuki Kobayashi, Soichiro Muneta, and Koichiro Ishimori, Biochemistry, 2017, 56, 2425-2434<\/a><\/h2><p>CyaY\u306f\u751f\u4f53\u5206\u5b50\u306e\u9178\u5316\u9084\u5143\u4e2d\u5fc3\u3067\u3042\u308b\u9244-\u786b\u9ec4\u30af\u30e9\u30b9\u30bf\u30fc\u3084\u30d8<wbr \/>\u30e0\u306e\u751f\u5408\u6210\u7cfb\u306b\u9244\u3092\u8f38\u9001\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3001<wbr \/>\u5fae\u751f\u7269\u304b\u3089\u30d2\u30c8\u307e\u3067\u307b\u307c\u5168\u3066\u306e\u751f\u7269\u306b\u5171\u901a\u306b\u5b58\u5728\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e<wbr \/>\u4e00\u3064\u3067\u3042\u308b\u3002<wbr \/>\u30d2\u30c8\u306e\u76f8\u540c\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u30d5\u30e9\u30bf\u30ad\u30b7\u30f3\u306b\u7570\u5e38\u304c\u8d77\u3053\u308b\u3068\u30d5\u30ea\u30fc<wbr \/>\u30c9\u30e9\u30a4\u30d2\u904b\u52d5\u5931\u8abf\u75c7\u3092\u767a\u75c7\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u672c\u7814\u7a76\u3067\u306fC<wbr \/>yaY\u306f\u9244\u3060\u3051\u306f\u306a\u304f\u3001\u30d8\u30e0\u4e8c\u5206\u5b50\u3068\u3082\u7d50\u5408\u3057\u3001<wbr \/>\u305d\u308c\u306b\u3088\u308a\u5358\u91cf\u4f53\u304b\u3089\u4e09\u91cf\u4f53\u306b\u5909\u5316\u3057\u3001<wbr \/>\u9244\u306e\u89aa\u548c\u6027\u304c\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001<wbr \/>\u9244\u3092\u542b\u3080\u88dc\u56e0\u5b50\u306e\u751f\u5408\u6210\u306f\u9244\u3060\u3051\u3067\u306f\u306a\u304f\u3001<wbr \/>\u30d8\u30e0\u306e\u6fc3\u5ea6\u306b\u3082\u4f9d\u5b58\u3057\u3001<wbr \/>\u8abf\u7bc0\u3055\u308c\u308b\u3068\u3044\u3046\u3053\u308c\u307e\u3067\u77e5\u3089\u308c\u3066\u3044\u306a\u3044\u5236\u5fa1\u6a5f\u69cb\u304c\u5b58\u5728\u3059\u308b\u3053\u3068<wbr \/>\u304c\u793a\u3055\u308c\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-CyaY.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-1139 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-CyaY.gif\" alt=\"TOC-CyaY\" width=\"500\" height=\"207\" \/><\/a><\/p><h2><a href=\"http:\/\/pubs.rsc.org\/en\/content\/articlelanding\/2017\/dt\/c7dt00121e#!divAbstract\">Iron Chelators Inhibit the Heme-Degradation Reaction by HutZ form <em>Vibrio cholerae,\u00a0<\/em>Nobuhiko Dojun, Yukari Sekine, Koichiro Ishimori, Takeshi Uchida, <em>Dalton Trans.<\/em>, 2017, <strong>46<\/strong>, 5147<\/a><\/h2><p>\u75c5\u539f\u83cc\u306f\u5897\u6b96\u306e\u305f\u3081\u9244\u7372\u5f97\u3092\u5fc5\u8981\u3068\u3059\u308b\u3002\u5bbf\u4e3b\u3067\u3042\u308b\u4eba\u306e\u4f53\u5185\u306b\u5b58\u5728\u3059\u308b\u9244\u306e\u591a\u304f\u306f\u3001\u8d64\u8840\u7403\u306b\u5b58\u5728\u3059\u308b\u30d8\u30e2\u30b0\u30ed\u30d3\u30f3\u4e2d\u306b\u9244-\u30dd\u30eb\u30d5\u30a3\u30ea\u30f3\u932f\u4f53\u3067\u3042\u308b\u30d8\u30e0\u3068\u3057\u3066\u5b58\u5728\u3059\u308b\u3002\u30b3\u30ec\u30e9\u83cc\u3067\u306f\u30d8\u30e0\u306e\u7372\u5f97\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5229\u7528\u3057\u30d8\u30e0\u3092\u7372\u5f97\u3057\u3001\u30d8\u30e0\u306e\u5206\u89e3\u9175\u7d20\u3067\u3042\u308bHutZ\u304c\u30d8\u30e0\u3092\u5206\u89e3\u3057\u9244\u3092\u53d6\u308a\u51fa\u3059\u3002\u4eca\u56de\u3001\u3053\u306eHutZ\u306b\u3088\u308b\u30d8\u30e0\u5206\u89e3\u53cd\u5fdc\u304c\u30ad\u30ec\u30fc\u30c8\u5264\u306b\u3088\u308a\u6291\u5236\u3055\u308c\u308b\u3053\u3068\u3092\u767a\u898b\u3057\u305f\u3002\u5206\u5149\u5b66\u7684\u624b\u6cd5\u3092\u7528\u3044\u3066\u30ad\u30ec\u30fc\u30c8\u5264\u304c\u6d3b\u6027\u90e8\u4f4d\u8fd1\u508d\u306e\u6c34\u5206\u5b50\u306e\u69cb\u9020\u3092\u5909\u3048\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u9178\u7d20\u7d50\u5408\u4f53\u306e\u6c34\u9178\u5316\u904e\u7a0b\u3092\u6291\u5236\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3002HutZ\u306e\u9175\u7d20\u6d3b\u6027\u306e\u963b\u5bb3\u306f\u30b3\u30ec\u30e9\u83cc\u306e\u9244\u7372\u5f97\u306e\u6291\u5236\u306b\u3064\u306a\u304c\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3053\u3068\u304b\u3089\u3001\u30ad\u30ec\u30fc\u30c8\u80fd\u3092\u6301\u3064\u5316\u5408\u7269\u304c\u30b3\u30ec\u30e9\u83cc\u3092\u542b\u3080\u591a\u304f\u306e\u75c5\u539f\u83cc\u306e\u6cbb\u7642\u85ac\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u793a\u3055\u308c\u305f\u3002<\/p><p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter  wp-image-1113 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528.png\" alt=\"iron chelator HP\u7528\" width=\"365\" height=\"223\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528.png 1055w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-300x183.png 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-1024x627.png 1024w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-690x422.png 690w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-540x330.png 540w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-340x208.png 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-223x136.png 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/iron-chelator-HP\u7528-124x75.png 124w\" sizes=\"(max-width: 365px) 100vw, 365px\" \/><\/p><h2><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0006291X17300438\">Dual role of the active-center cysteine in human peroxiredoxin 1: Peroxidase activity and heme binding, Y. Watanabea, K. Ishimori, and T. Uchida, Biochem Biophys Res Commun., 483, 930 (2017)<\/a><\/h2><p>\u30da\u30eb\u30aa\u30ad\u30b7\u30ec\u30c9\u30ad\u30b7\u30f3 (PRX1)\u306f\u4e8c\u3064\u306eCys\u9593\u3067S-<wbr \/>S\u7d50\u5408\u306e\u5f62\u6210\u3068\u958b\u88c2\u3092\u7e70\u308a\u8fd4\u3059\u3053\u3068\u3067\u3001<wbr \/>\u904e\u9178\u5316\u6c34\u7d20\u3092\u9664\u53bb\u3059\u308b\u6297\u9178\u5316\u9175\u7d20\u3067\u3042\u308b\u4e00\u65b9\u3001<wbr \/>\u30d8\u30e0\u3068\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u308b\u304c\u3001<wbr \/>\u30d8\u30e0\u306e\u6a5f\u80fd\u306f\u4e0d\u660e\u3067\u3042\u3063\u305f\u3002\u4eca\u56de\u3001\u5909\u7570\u4f53\u3092\u7528\u3044\u305f\u7814\u7a76\u304b\u3089\u3001<wbr \/>\u30d8\u30e0\u306fS-S\u7d50\u5408\u3092\u5f62\u6210\u3059\u308bCys52\u306b\u7d50\u5408\u3059\u308b\u305f\u3081\u3001<wbr \/>\u30d8\u30e0\u306e\u7d50\u5408\u306b\u3088\u308a\u904e\u9178\u5316\u6c34\u7d20\u306e\u9664\u53bb\u80fd\u304c\u6d88\u5931\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>\u3057\u304b\u3057\u3001<wbr \/>\u904e\u9178\u5316\u6c34\u7d20\u306b\u3088\u308b\u9178\u5316\u6d3b\u6027\u3084\u30d8\u30e0\u306e\u5206\u89e3\u304c\u6291\u5236\u3055\u308c\u308b\u3053\u3068\u304b\u3089\u3001<wbr \/>PRX1\u306b\u306f\u7d30\u80de\u8cea\u5185\u306e\u904a\u96e2\u306e\u30d8\u30e0\u3092\u4fdd\u8b77\u3057\u3001<wbr \/>\u8102\u8cea\u306e\u9178\u5316\u306a\u3069\u306e\u526f\u53cd\u5fdc\u3092\u6291\u5236\u3057\u3001<wbr \/>\u30d8\u30e0\u3092\u5fc5\u8981\u3068\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5b89\u5168\u306b\u8f38\u9001\u3059\u308b\u6a5f\u80fd\u304c\u3042\u308b\u3053\u3068\u304c\u793a<wbr \/>\u5506\u3055\u308c\u305f\u3002<\/p><p style=\"text-align: center;\"><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter  wp-image-1118 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1.jpeg\" alt=\"TOC-PRX1\" width=\"350\" height=\"138\" srcset=\"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1.jpeg 500w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1-300x118.jpeg 300w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1-340x133.jpeg 340w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1-223x87.jpeg 223w, https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/TOC-PRX1-124x48.jpeg 124w\" sizes=\"(max-width: 350px) 100vw, 350px\" \/><\/a><\/p><h2><a href=\"http:\/\/www.jbc.org\/content\/291\/29\/15320.short\">Energetic Mechanism of Cytochrome <em>c\u00a0<\/em>&#8211; Cytochrome <em>c\u00a0<\/em>Oxidase Electron Transfer Complex Formation under Turnover Conditions Revealed by Mutational Effects and Docking Simulation,\u00a0W. Sato, S. Hitaoka, K. Inoue, M. Imai, T. Saio, T. Uchida, K. Shinzawa-Itoh, S. Yoshikawa, K. Yoshizawa, and K. Ishimori, <i>J.Biol.Chem.<\/i>, 291, 15320-15331(2016)<\/a><\/h2><p>\u7d30\u80de\u5185\u30a8\u30cd\u30eb\u30ae\u30fc\u901a\u8ca8\u306b\u4f8b\u3048\u3089\u308c\u308bATP\u306f\u3001\u7cd6\u306e\u5206\u89e3\u306a\u3069\u3067\u751f\u3058\u305f\u96fb\u5b50\u304c\u7d30\u80de\u5c0f\u5668\u5b98\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u5185\u819c\u306b\u57cb\u3081\u8fbc\u307e\u308c\u3066\u3044\u308b\u4e00\u9023\u306e\u547c\u5438\u9396\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4ecb\u3057\u3066\u9178\u7d20\u5206\u5b50\u306b\u307e\u3067\u4f1d\u9054\u3055\u308c\u308b\u300c\u96fb\u5b50\u4f1d\u9054\u7cfb\u300d\u306e\u99c6\u52d5\u306b\u3088\u3063\u3066\u5408\u6210\u3055\u308c\u308b\u3002\u3053\u306e\u751f\u7406\u7684\u306b\u91cd\u8981\u306a\u96fb\u5b50\u4f1d\u9054\u7cfb\u306b\u304a\u3044\u3066\u3001\u30b7\u30c8\u30af\u30ed\u30e0<i>c<\/i>\u9178\u5316\u9175\u7d20(C<i>c<\/i>O)\u306f\u9178\u7d20\u5206\u5b50\u3092\u6c34\u5206\u5b50\u306b\u9084\u5143\u3059\u308b\u3053\u3068\u3067\u96fb\u5b50\u4f1d\u9054\u3092\u7d42\u7d50\u3055\u305b\u308b\u5f79\u5272\u3092\u62c5\u3044\u3001\u305d\u306e\u53cd\u5fdc\u306b\u8981\u3059\u308b\u96fb\u5b50\u306f\u30b7\u30c8\u30af\u30ed\u30e0<i>c<\/i>(Cyt <i>c<\/i>)\u304b\u3089\u4f1d\u9054\u3055\u308c\u308b\u3002\u672c\u7814\u7a76\u3067\u306fCyt <i>c<\/i>\u304b\u3089C<i>c<\/i>O\u3078\u96fb\u5b50\u4f1d\u9054\u3059\u308b\u969b\u306b\u5f62\u6210\u3055\u308c\u308bCyt <i>c<\/i>-C<i>c<\/i>O\u8907\u5408\u4f53\u306b\u7740\u76ee\u3057\u3001\u3053\u306e\u8907\u5408\u4f53\u306e\u5f62\u6210\u306b\u5bc4\u4e0e\u3059\u308b\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306b\u3064\u3044\u3066\u691c\u8a0e\u3059\u308b\u305f\u3081\u3001\u5909\u7570\u4f53\u6d3b\u6027\u6e2c\u5b9a\u4e26\u3073\u306b\u4f1a\u5408\u306e\u30c9\u30c3\u30ad\u30f3\u30b0\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3063\u305f\u3002\u305d\u306e\u7d50\u679c\u3001Cyt <i>c<\/i>\u306e\u591a\u304f\u306eLys\u6b8b\u57fa\u306b\u8d77\u56e0\u3059\u308b\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\u306e\u8907\u5408\u4f53\u5f62\u6210\u3078\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u7684\u5bc4\u4e0e\u306f\u305d\u306e\u5927\u90e8\u5206\u304c\u8131\u6eb6\u5a92\u548c\u306e\u52b9\u679c\u306b\u3088\u3063\u3066\u76f8\u6bba\u3055\u308c\u308b\u305f\u3081\u306b\u6975\u3081\u3066\u4e4f\u3057\u304f\u3001\u8907\u5408\u4f53\u306e\u5b89\u5b9a\u5316\u306f\u4e3b\u306b\u5c11\u6570\u306e\u758e\u6c34\u6027\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u306b\u3088\u308b\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306b\u8d77\u56e0\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u65b0\u305f\u306b\u793a\u3055\u308c\u305f\u3002\u3053\u306e\u3088\u3046\u306aCyt <i>c<\/i>-C<i>c<\/i>O\u9593\u306b\u5f62\u6210\u3055\u308c\u308b\u7279\u7570\u7684\u4e14\u3064\u5f31\u3044\u758e\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u9593\u3067\u8fc5\u901f\u306a\u7d50\u5408\uff0d\u89e3\u96e2\u3092\u53ef\u80fd\u306b\u3059\u308b\u70b9\u3067Cyt <i>c<\/i>\u304b\u3089C<i>c<\/i>O\u3078\u306e\u6052\u5e38\u7684\u306a\u96fb\u5b50\u4f1d\u9054\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2018\/03\/JBC_Abstract.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-thumbnail wp-image-1318 colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2018\/03\/JBC_Abstract-150x150.png\" alt=\"JBC_Abstract\" width=\"150\" height=\"150\" \/><\/a>\u00a0<\/p><p>&nbsp;<\/p><h2><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.biochem.5b01273\">Cytoplasmic Heme-Binding Protein (HutX) from Vibrio cholerae Is an Intracellular Heme Transport Protein for\u00a0the Heme-Degrading Enzyme, HutZ, Y. Sekine, T. Tanzawa, Y. Tanaka, K. Ishimori, and T. Uchida, Biochemistry,\u00a055, 884 (2016)<\/a><\/h2><div>\u3053\u308c\u307e\u3067\u306e\u7814\u7a76\u306b\u3088\u308a\u3001<wbr \/>HutZ\u304c\u30b3\u30ec\u30e9\u83cc\u306e\u7d30\u80de\u8cea\u306b\u5b58\u5728\u3059\u308b\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u3042\u308b\u3053\u3068\u3092<wbr \/>\u660e\u3089\u304b\u306b\u3057\u3066\u304d\u305f\u00a0(Uchida et al., Chem. Commun., 2012)\u3002HutZ\u907a\u4f1d\u5b50\u306fHutX, HutW\u3068\u30aa\u30da\u30ed\u30f3\u3092\u5f62\u6210\u3057\u3066\u304a\u308a\u3001<wbr \/>\u5927\u8178\u83cc\u306b\u5b58\u5728\u3059\u308bHutX\u306e\u76f8\u540c\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30d8\u30e0\u3068\u7d50\u5408\u3059\u308b\u3068\u3044<wbr \/>\u3046\u5831\u544a\u304c\u3042\u308b\u3053\u3068\u304b\u3089\u3001<wbr \/>HutX\u304cHutZ\u306b\u30d8\u30e0\u3092\u8f38\u9001\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u3068\u4e88\u60f3\u3057\u3001<wbr \/>\u767a\u73fe\u7cfb\u3092\u69cb\u7bc9\u3057\u3001\u3078\u30e0\u306e\u8f38\u9001\u3092\u89b3\u6e2c\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001<wbr \/>HutX\u306fTyr90\u3092\u914d\u4f4d\u5b50\u3068\u3057\u3001\u30d8\u30e0\u3068\u7d50\u5408\u3057\u3001<wbr \/>\u305d\u306e\u89aa\u548c\u6027\u306fHutZ\u3068\u307b\u307c\u540c\u7a0b\u5ea6\u3067\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002<wbr \/>HutX\u306fHutZ\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001<wbr \/>\u30d8\u30e0\u306e\u89aa\u548c\u6027\u3092\u8abf\u7bc0\u3057\u3001<wbr \/>\u4e00\u65b9\u7684\u306bHutX\u304b\u3089HutZ\u3078\u30d8\u30e0\u304c\u8f38\u9001\u3055\u308c\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u3001<wbr \/>\u7d30\u80de\u8cea\u5185\u306e\u30d8\u30e0\u8f38\u9001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3002<\/div><div><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/bi-2015-01273d_0009.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1119 aligncenter colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/bi-2015-01273d_0009.gif\" alt=\"bi-2015-01273d_0009\" width=\"350\" height=\"167\" \/><\/a><\/div><h2>Investigation of the redox-dependent modulation of structure and dynamics in human cytochrome <em>c,\u00a0<\/em>M. Imai, T. Saio, H. Kumeta, T. Uchida, F. Inagaki and K. Ishimori,<em> Biochem.Biophys.Res.Commun.,<\/em> 469(4), 978 (2016)<\/h2><p>\u7d30\u80de\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u5de5\u5834\u3067\u3042\u308b\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3001\u305d\u3053\u3067\u306f\u7cd6\u985e\u306e\u5206\u89e3\u306b\u3088\u3063\u3066\u5f97\u3089\u3048\u305f\u96fb\u5b50\u304c\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u5185\u819c\u306b\u57cb\u3081\u8fbc\u307e\u308c\u305f\u8907\u6570\u306e\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u9593\u3067\u4f1d\u9054\u3055\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066 ATP\u5408\u6210\u304c\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u79c1\u305f\u3061\u306f\u3053\u306e\u96fb\u5b50\u4f1d\u9054\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u89e3\u660e\u3092\u76ee\u6307\u3057\u3001\u7279\u306b\u96fb\u5b50\u4f1d\u9054\u306e\u6700\u7d42\u6bb5\u968e\u3092\u62c5\u3046\u96fb\u5b50\u4f1d\u9054\u30d8\u30e0\u30bf\u30f3\u30d1\u30af\u8cea\u306eCytochrome <i>c<\/i> (Cyt <i>c<\/i>) \u3068\u5de8\u5927\u306a\u5206\u5b50\u91cf\u3067\u8907\u96d1\u306a\u69cb\u9020\u3092\u3082\u3064\u819c\u7d50\u5408\u9178\u5316\u9175\u7d20Cyt <i>c<\/i> Oxidase (C<i>c<\/i>O) \u306e\u9593\u3067\u306e\u96fb\u5b50\u4f1d\u9054\u306b\u3064\u3044\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u96fb\u5b50\u3092\u6301\u3063\u305f\u9084\u5143\u72b6\u614b\u306eCyt <i>c<\/i>\u304cC<i>c<\/i>O\u306b\u9078\u629e\u7684\u306b\u7d50\u5408\u3057\u3001\u96fb\u5b50\u3092\u53d7\u3051\u6e21\u3057\u3066\u9178\u5316\u72b6\u614b\u3068\u306a\u3063\u305fCyt <i>c<\/i>\u306f\u901f\u3084\u304b\u306bC<i>c<\/i>O\u304b\u3089\u89e3\u96e2\u3059\u308b\u3053\u3068\u3067\u3001\u9178\u7d20\u5206\u5b50\u3092\u6c34\u306b\u9084\u5143\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u8a73\u7d30\u306a\u5236\u5fa1\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u672a\u3060\u660e\u3089\u304b\u306b\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u79c1\u305f\u3061\u306f\u3001Cyt <i>c<\/i> \u3068C<i>c<\/i>O\u306e\u7d50\u5408\u304c\u3069\u306e\u3088\u3046\u306b\u5236\u5fa1\u3055\u308c\u3001\u3069\u306e\u3088\u3046\u306b\u3057\u3066\u52b9\u7387\u7684\u306a\u96fb\u5b50\u4f1d\u9054\u304c\u53ef\u80fd\u306b\u306a\u3063\u3066\u3044\u308b\u306e\u304b\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u305f\u3081\u306b\u3001\u6eb6\u6db2NMR\u6cd5\u3092\u7528\u3044\u3066Cyt <i>c<\/i>\u306e\u7acb\u4f53\u69cb\u9020\u3068\u305d\u306e\u30c0\u30a4\u30ca\u30df\u30af\u30b9\u3092\u9178\u5316\u72b6\u614b\u3001\u9084\u5143\u72b6\u614b\u306e\u4e21\u65b9\u306b\u3064\u3044\u3066\u89e3\u6790\u3057\u307e\u3057\u305f\u3002Cyt <i>c<\/i>\u306e\u7acb\u4f53\u69cb\u9020\u89e3\u6790\u306e\u7d50\u679c\u3001C<i>c<\/i>O\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u9762\u306b\u52a0\u3048\u3001\u305d\u306e\u53cd\u5bfe\u5074\u306b\u3082Cyt <i>c<\/i>\u306e\u9178\u5316\u9084\u5143\u72b6\u614b\u306b\u4f9d\u5b58\u3057\u305f\u7acb\u4f53\u69cb\u9020\u5909\u5316\u304c\u89b3\u6e2c\u3055\u308c\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u30c0\u30a4\u30ca\u30df\u30af\u30b9\u306e\u9762\u304b\u3089\u306f\u3001C<i>c<\/i>O\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u9762\u3088\u308a\u3082\u3001\u3080\u3057\u308d\u305d\u306e\u53cd\u5bfe\u5074\u306e\u9818\u57df\u304c\u67d4\u8edf\u306a\u69cb\u9020\u3067\u3001\u69cb\u9020\u7684\u306a\u300c\u63fa\u3089\u304e\u300d\u304c\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u76f8\u4e92\u4f5c\u7528\u9762\u306b\u304a\u3051\u308b\u69cb\u9020\u5909\u5316\u306fC<i>c<\/i>O\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306e\u5236\u5fa1\u306b\u91cd\u8981\u3067\u3042\u308b\u3068\u5bb9\u6613\u306b\u60f3\u50cf\u3067\u304d\u307e\u3059\u304c\u3001\u305d\u306e\u53cd\u5bfe\u5074\u3067\u89b3\u6e2c\u3055\u308c\u305f\u69cb\u9020\u5909\u5316\u3068\u300c\u63fa\u3089\u304e\u300d\u306f\u4e88\u60f3\u5916\u3067\u8208\u5473\u6df1\u304f\u3001\u3053\u308c\u3089\u306e\u69cb\u9020\u7684\u7279\u6027\u304c\u3069\u306e\u3088\u3046\u306bC<i>c<\/i>O\u3068\u306e\u76f8\u4e92\u4f5c\u7528\u306e\u5236\u5fa1\u3001\u3055\u3089\u306b\u306f\u96fb\u5b50\u306e\u4f1d\u9054\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u306e\u304b\u3001\u4eca\u5f8c\u306e\u7814\u7a76\u3067\u306e\u89e3\u660e\u304c\u671f\u5f85\u3055\u308c\u307e\u3059\u3002\uff08T. S.\uff09<\/p><h2><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.biochem.5b00952?src=recsys\">\u00a0A Dye-Decolorizing Peroxidase from Vibrio cholerae, T. Uchida, M. Sasaki, Y. Tanaka, and K. Ishimori,\u00a0Biochemistry, 54, 6610 (2015)<\/a><\/h2><p>\u8272\u7d20\u5206\u89e3\u578b\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc\u30bc(DyP)\u306f\u3001<wbr \/>\u30a2\u30f3\u30c8\u30e9\u30ad\u30ce\u30f3\u7cfb\u8272\u7d20\u3092\u904e\u9178\u5316\u6c34\u7d20\u306b\u3088\u308a\u5206\u89e3\u3059\u308b\u30da\u30eb\u30aa\u30ad\u30b7\u30c0\u30fc<wbr \/>\u30bc\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u4e00\u3064\u3067\u3042\u308b\u3002\u4eca\u56de\u3001<wbr \/>\u30b3\u30ec\u30e9\u83cc\u306eDyP\u306e\u7d50\u6676\u69cb\u9020\u89e3\u6790 (PDB\u30b3\u30fc\u30c9: 5DE0) \u3068\u5909\u7570\u4f53\u306e\u4f5c\u6210\u3092\u3082\u3068\u306b\u3001\u53cd\u5fdc\u6a5f\u69cb\u3092\u691c\u8a0e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001<wbr \/>\u30d8\u30e0\u3067\u767a\u751f\u3057\u305f\u30e9\u30b8\u30ab\u30eb\u304c\u4e8c\u91cf\u4f53\u754c\u9762\u306b\u5b58\u5728\u3059\u308bTyr129\u3068T<wbr \/>yr235\u306b\u79fb\u52d5\u3057\u3001\u8272\u7d20\u3092\u5206\u89e3\u3059\u308b\u3001<wbr \/>\u3068\u3044\u3046\u53cd\u5fdc\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u305f\u3002<\/p><p><a href=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/bi-2015-00952n_0010.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1120 aligncenter colorbox-19\" src=\"http:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-content\/uploads\/2014\/03\/bi-2015-00952n_0010.gif\" alt=\"bi-2015-00952n_0010\" width=\"500\" height=\"168\" \/><\/a><\/p><h2>Unique Heme Environmental Structures in Heme-regulated Proteins Using Heme as the Signaling Molecule, K. Ishimori and Y. Watanabe,\u00a0<em>Chem.Lett.,\u00a0<\/em>43(11), 1680 (2014)<\/h2><p>\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u88dc\u6b20\u5206\u5b50\u65cf\u3068\u3057\u3066\u6700\u3082\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u3082\u306e\u306e\u4e00\u3064\u306b\u3001\u9244\u3068\u30dd\u30eb\u30d5\u30a3\u30ea\u30f3\u3068\u3088\u3070\u308c\u308b\u74b0\u72b6\u5206\u5b50\u304b\u3089\u306a\u308b\u932f\u4f53\u300c\u30d8\u30e0\u300d\u304c\u6319\u3052\u3089\u308c\u308b\u3002\u30d8\u30e0\u306e\u6a5f\u80fd\u3068\u3057\u3066\u306f\u3001\u30d8\u30e2\u30b0\u30ed\u30d3\u30f3\u306b\u3088\u308b\u9178\u7d20\u904b\u642c(\u9178\u7d20\u5206\u5b50\u306e\u7d50\u5408)\u3084\u30b7\u30c8\u30af\u30ed\u30e0<i>c<\/i>\u306b\u3088\u308b\u96fb\u5b50\u4f1d\u9054(\u9178\u5316\u9084\u5143\u4e2d\u5fc3)\u306b\u4ee3\u8868\u3055\u308c\u308b\u3088\u3046\u306a\u6d3b\u6027\u4e2d\u5fc3\u3068\u3057\u3066\u306e\u6a5f\u80fd\u306f\u5e83\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u3002\u4e00\u65b9\u3001\u6700\u8fd1\u306e\u7814\u7a76\u306b\u3088\u308a\u3001\u3053\u306e\u3088\u3046\u306a\u6d3b\u6027\u4e2d\u5fc3\u3068\u3057\u3066\u306e\u6a5f\u80fd\u306b\u52a0\u3048\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u3092\u5236\u5fa1\u3059\u308b\u30ea\u30ac\u30f3\u30c9\u3001\u3059\u306a\u308f\u3061\u30b7\u30b0\u30ca\u30eb\u5206\u5b50\u3068\u3057\u3066\u3082\u6a5f\u80fd\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u3066\u304d\u305f\u3002\u30d8\u30e0\u3092\u30b7\u30b0\u30ca\u30eb\u5206\u5b50\u3068\u3057\u3066\u7528\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30d0\u30af\u30c6\u30ea\u30a2\u304b\u3089\u30d2\u30c8\u307e\u3067\u591a\u69d8\u306a\u751f\u7269\u7a2e\u306b\u5b58\u5728\u3057\u3001\u305d\u306e\u30d8\u30e0\u7d50\u5408\u90e8\u4f4d\u3068\u3057\u3066\u3001\u5171\u901a\u3059\u308b\u7279\u5fb4\u7684\u306a\u30a2\u30df\u30ce\u9178\u914d\u5217\u3092\u6301\u3063\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\u3002\u307e\u305f\u3001\u305d\u306e\u6a5f\u80fd\u3082\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u304b\u3089\u30a4\u30aa\u30f3\u30c1\u30e3\u30cd\u30eb\u3001\u6982\u65e5\u30ea\u30ba\u30e0\u306a\u3069\u591a\u5c90\u306b\u308f\u305f\u308a\u3001\u30d8\u30e0\u306e\u751f\u7406\u6a5f\u80fd\u306b\u304a\u3051\u308b\u65b0\u305f\u306a\u5074\u9762\u3068\u3057\u3066\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u3002\u672c\u7dcf\u8aac\u3067\u306f\u3001\u30d8\u30e0\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u69cb\u9020\u89e3\u6790\u306b\u5f37\u529b\u306a\u624b\u6cd5\u3067\u3042\u308b\u5206\u5149\u5b66\u3092\u7528\u3044\u3066\u306a\u3055\u308c\u305f\u7814\u7a76\u3092\u4e2d\u5fc3\u306b\u3001\u30d8\u30e0\u306b\u3088\u3063\u3066\u6d3b\u6027\u5236\u5fa1\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u201dHeme-regulated protein\u201d\u306e\u7279\u7570\u306a\u30d8\u30e0\u914d\u4f4d\u74b0\u5883\u3084\u305d\u306e\u5468\u8fba\u69cb\u9020\u306b\u3064\u3044\u3066\u6700\u65b0\u306e\u8b70\u8ad6\u3092\u307e\u3068\u3081\u305f\u3002(Y.W.)<\/p><p>&nbsp;<\/p><h2><a href=\"http:\/\/pubs.rsc.org\/en\/Content\/ArticleLanding\/2012\/CC\/c2cc31147j#!divAbstract\">A heme degradation enzyme, HutZ, from Vibrio cholerae, T. Uchida, Y. Sekine, T. Matsui, M. Ikeda-Saito, and K. Ishimori, <em>Chem. Commun.<\/em>, <strong>48<\/strong>, 6741 (2012)<\/a><\/h2><p>\u30b3\u30ec\u30e9\u306f\u4ee3\u8868\u7684\u306a\u611f\u67d3\u75c7\u306e\u4e00\u3064\u3067\u3042\u308a\u3001\u7d4c\u53e3\u6442\u53d6\u306b\u3088\u308a\u4f53\u5185\u306b\u4fb5\u5165\u3057\u305f\u30b3\u30ec\u30e9\u83cc\u304c\u8178\u5185\u3067\u5897\u6b96\u3057\u3001\u305d\u308c\u3089\u304c\u7523\u751f\u3059\u308b\u30b3\u30ec\u30e9\u6bd2\u7d20\u306b\u3088\u308a\u3001\u4e0b\u75e2\u3084\u5614\u5410\u306a\u3069\u3092\u767a\u75c7\u3059\u308b\u3002\u83cc\u306f\u30a8\u30cd\u30eb\u30ae\u30fc\u7523\u751f\u306b\u9244\u3092\u542b\u3080\u86cb\u767d\u8cea\u3092\u5229\u7528\u3059\u308b\u3053\u3068\u304b\u3089\u3001\u5897\u6b96\u306b\u9244\u30a4\u30aa\u30f3\u3092\u5fc5\u8981\u3068\u3059\u308b\u3002\u4eba\u4f53\u306b\u611f\u67d3\u3057\u305f\u83cc\u4f53\u306f\u3001\u5bbf\u4e3b\u304b\u3089\u9244\u3092\u7372\u5f97\u3059\u308b\u305f\u3081\u3001\u305d\u306e\u7372\u5f97\u6a5f\u69cb\u306e\u89e3\u660e\u306f\u75c5\u539f\u83cc\u306e\u611f\u67d3\u9632\u6b62\u7b49\u306b\u3064\u306a\u304c\u308b\u91cd\u8981\u306a\u30c6\u30fc\u30de\u3067\u3042\u308b\u3002\u4f53\u5185\u306b\u5b58\u5728\u3059\u308b\u9244\u306e\u7d0470\uff05\u306f\u30d8\u30e2\u30b0\u30ed\u30d3\u30f3\u4e2d\u306b\u30d8\u30e0\u3068\u3057\u3066\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u30d8\u30e0\u304c\u30b3\u30ec\u30e9\u83cc\u306e\u4e3b\u8981\u306a\u9244\u6e90\u3068\u306a\u308b\u3002\u30b3\u30ec\u30e9\u83cc\u306fHut\u3068\u547c\u3070\u308c\u308b\u4e00\u7fa4\u306e\u86cb\u767d\u8cea\u3092\u5229\u7528\u3057\u3001\u30d8\u30e0\u3092\u7d30\u83cc\u5185\u306b\u53d6\u308a\u8fbc\u3080\u3002\u907a\u4f1d\u5b50\u89e3\u6790\u306e\u7d50\u679c\u3001HutZ\u3068\u547c\u3070\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3067\u3042\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u305f\u3002\u305d\u3053\u3067\u3001HutZ\u306e\u767a\u73fe\u7cfb\u3092\u69cb\u7bc9\u3057\u3001\u305d\u306e\u30d8\u30e0\u5206\u89e3\u6d3b\u6027\u3092\u8abf\u3079\u305f\u3002HutZ\u306f\u30d2\u30c8\u304c\u3082\u3064\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u3068\u30a2\u30df\u30ce\u9178\u914d\u5217\u306b\u76f8\u540c\u6027\u304c\u306a\u3044\u306b\u3082\u95a2\u308f\u3089\u305a\u3001\u30d2\u30c8\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u306b\u7279\u5fb4\u7684\u306a\u30d9\u30eb\u30c9\u30d8\u30e0\u3068\u547c\u3070\u308c\u308b\u4e2d\u9593\u4f53\u3092\u7d4c\u7531\u3057\u3001\u30d8\u30e0\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u3055\u3089\u306b\u3001\u6d3b\u6027\u4e2d\u5fc3\u8fd1\u508d\u306b\u4ed6\u306e\u30d8\u30e0\u5206\u89e3\u9175\u7d20\u306b\u5b58\u5728\u3057\u306a\u3044\u6c34\u7d20\u7d50\u5408\u306e\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u304c\u5b58\u5728\u3057\u3001\u305d\u306e\u6c34\u7d20\u7d50\u5408\u306e\u5f37\u5ea6\u3092\u5909\u5316\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u9175\u7d20\u6d3b\u6027\u3092\u5236\u5fa1\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u3057\u305f\u3002<\/p><p>\u00a0<img decoding=\"async\" class=\"aligncenter colorbox-19\" src=\"https:\/\/gm1.ggpht.com\/bU394AdnfKkzBI8WgiW97BJ4HAYW6huZzit4XkY6b3lpdByLoOtoge4exraUSuxtJE-wjKQ-9v0MdWF2gQH8qBBiV1pricInu8CZwtiNCNuwD9U2-8iDekwHqjDjlfPUPsC7qBHqT3sd5-b7FaBnDWO4EnTsGdcQ8pNUK0JcRkGviMBFVxYthOevDPbksNMGA3ysmaz5fppd9SJ2mOcVY3qx_LZ9rSnMyA6NQynEqNn97U9UOsPgUaBfDht_GHNZGgpW7wxRqaWGqlItbTJ8QR0nfHdvdb7Rfr_aSVs6Ncp3EUM-49qMv3po3QTk-oI110Mpmrs7wVl-7jlWk0CselTrTxIwqcT76Rxq6HoPxukyvxLgHB1vUOfNZQ9DD0B5jHKXD4C4a8brT2BI_jY5UizFtyJRSqRIM0HEIP5zgDElfqURyYjaxfWZIlo0lNBP31vOGyq8acgb-3kl6-ZejkI3SsmOha0PHxIWD7wXI51njorqq-k3mHZqqgGfgKKOrZEZwwWdgl5ebdwoSY0kFfYFyub4nrt2P4EGu8FFeQCcDIYN98gRJxs_KEmI1dajVjOcqMIT-uRSM3fQAto1EI8n6GruRAdaEK1Zr6MW0lddiPVlNjAEPZPNlQ1KXNx7aB8MHNf4gbrRDe7sS6UXTbuxTjqDBLOr7mGrnN0LAhRa-10Ra_JEXrMSBP00g-9eLIm2oWWiqxDA7a63S1drSifIa6bksg=w756-h248-l75-ft\" alt=\"\" \/><\/p><h2>Structural features of small benzene clusters (C<sub>6<\/sub>H<sub>6<\/sub>)n (n\u226430) as investigated with the all-atom OPLS potential, H. Takeuchi, <em>J. Phys. Chem. A<\/em>, <strong>116<\/strong>, 10172 (2012)<\/h2><p>\u6700\u3082\u5358\u7d14\u306a\u82b3\u9999\u65cf\u70ad\u5316\u6c34\u7d20\u30af\u30e9\u30b9\u30bf\u30fc\u3067\u3042\u308b\u30d9\u30f3\u30bc\u30f3\u30af\u30e9\u30b9\u30bf\u30fc\u306e\u69cb\u9020\u306b\u95a2\u3057\u3066\u7814\u7a76\u306f\u5c11\u3057\u3057\u304b\u9032\u3093\u3067\u3044\u306a\u3044\u3002\u672c\u8ad6\u6587\u3067\u306f\u3001OPLS\u30dd\u30c6\u30f3\u30b7\u30e3\u30eb\u3092\u7528\u3044\u3066\u5f97\u3089\u308c\u305f\u69cb\u9020\u3092\u5831\u544a\u3059\u308b\u3002\u6700\u5b89\u5b9a\u69cb\u9020\u3068\u591a\u304f\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u306e\u4f4e\u3044\u69cb\u9020\u3092\u81ea\u4f5c\u306e\u69cb\u9020\u6442\u52d5\u30d2\u30e5\u30fc\u30ea\u30b9\u30c6\u30c3\u30af\u6cd5\u306b\u3088\u308b\u6c42\u3081\u305f\u3002\u30af\u30e9\u30b9\u30bf\u30fc\u69cb\u9020\u306e\u7279\u5fb4\u3068\u30af\u30e9\u30b9\u30bf\u30fc\u304c\u5927\u304d\u304f\u306a\u308b\u6642\u306e\u6210\u9577\u904e\u7a0b\u3092\u8abf\u3079\u305f\u3002\u305d\u306e\u969b\u306b\u306f\u3001\u5c40\u6240\u69cb\u9020\u89e3\u6790\u3068\u56de\u8ee2\u5b9a\u6570\u306b\u3088\u308b\u69cb\u9020\u7279\u5fb4\u62bd\u51fa\u3092\u63f4\u7528\u3057\u305f\u3002\u7b49\u65b9\u7684\u306a\u76f8\u4e92\u4f5c\u7528\u3092\u793a\u3059\u539f\u5b50\u306e\u30af\u30e9\u30b9\u30bf\u30fc\u3067\u306f\u30af\u30e9\u30b9\u30bf\u30fc \u5185\u306e\u57fa\u672c\u69cb\u9020\u304c\u4e8c\u5341\u9762\u4f53\u3067\u3042\u308b\u304c\u3001\u30d9\u30f3\u30bc\u30f3\u30af\u30e9\u30b9\u30bf\u30fc\u3067\u306f\u5206\u5b50\u9593\u76f8\u4e92\u4f5c\u7528\u306e\u7570\u65b9\u6027\u306b\u3088\u308a\u57fa\u672c\u69cb\u9020\u304c\u305d\u308c\u304b\u3089\u5927\u304d\u304f\u6b6a\u3093\u3067\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u30d9\u30f3\u30bc\u30f3\u306e\u4e8c\u4f53\u5206\u5e03\u95a2\u6570\u3092\u8abf\u3079\u308b\u3068\u3001\u5206\u5e03\u304c\u307b\u307c\u7b49\u65b9\u7684\u306a\u6db2\u4f53\u72b6\u614b\u3068\u7570\u306a\u308a\u3001\u30af\u30e9\u30b9\u30bf\u30fc\u3067\u306f\u304b\u306a\u308a\u7570\u65b9\u7684\u306a\u5206\u5e03\u3092\u793a\u3057\u305f\u3002\u4ee5\u524d\u306e\u8d85\u97f3\u901f\u30b8\u30a7\u30c3\u30c8\u4e2d\u3067\u306e\u30af\u30e9\u30b9\u30bf\u30fc\u306e\u5b9f\u9a13\u7d50 \u679c\u3092\u89e3\u91c8\u3059\u308b\u969b\u306b\u306f\u3001\u5206\u5b50\u306e\u4f4d\u7f6e\u304c\u5927\u304d\u304f\u7570\u306a\u308b\u69cb\u9020\u304c\u8907\u6570\u5b58\u5728\u3059\u308b\u3053\u3068\u306f\u306a\u304f\u3001\u5206\u5b50\u306e\u5411\u304d\u304c\u5909\u5316\u3057\u3066\u3044\u308b\u69cb\u9020\u304c\u5171\u5b58\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p><p>&nbsp;<\/p><h2>NMR Basis for Interprotein Electron Transfer Gating between Cytochrome <em>c<\/em> \u2013 Cytochrome <em>c<\/em> Oxidase,\u00a0K. Sakamoto, M. Kamiya, M. Imai, K. Shinzawa-Ito, T. Uchida, K. Kawano, S. Yoshikawa, and K. Ishimori,\u00a0<em>Proc. Natl. Acad. Sci. U.S.A.<\/em>, 108, 12271 (2011)<\/h2><p>\u591a\u304f\u306e\u751f\u7269\u306b\u3068\u3063\u3066\u751f\u547d\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b\u5fc5\u8981\u306a\u9178\u7d20\u306f\u7d30\u80de\u5185\u306b\u9001\u3089\u308c\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3068\u3044\u3046\u7d30\u80de\u5185\u5c0f\u5668\u5b98\u3067\u6c34\u306b\u5909\u63db\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u904e\u7a0b\u306b\u306f\u3001\u7d30\u80de\u5185\u3067\u7cd6\u985e\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\uff08\u89e3\u7cd6\u7cfb\uff09\u3067\u53d6\u308a\u3060\u3055\u308c\u305f\u96fb\u5b50\u304c\u5fc5\u8981\u3067\u3001\u3053\u306e\u96fb\u5b50\u304c\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u5185\u819c\u90e8\u5206\u306b\u57cb\u3081\u8fbc\u307e\u308c\u305f\u86cb\u767d\u8cea\u306e\u8907\u5408\u4f53\u9593\u3092\u4f1d\u9054\u3055\u308c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u5185\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u901a\u8ca8\u3068\u3088\u3070\u308c\u308bATP\u304c\u751f\u6210\u3057\u3001ATP\u306f\u751f\u7269\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u6e90\u3068\u3057\u3066\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u79c1\u305f\u3061\u306f\u3053\u306e\u3088\u3046\u306a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u5185\u306e\u96fb\u5b50\u4f1d\u9054\u7cfb\uff08\u547c\u5438\u9396\uff09\u306e\u4ed5\u7d44\u307f\u3092\u660e\u3089\u304b\u306b\u3057\u3066\u3001\u9178\u7d20\u547c\u5438\u751f\u7269\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u751f\u7523\u6a5f\u69cb\u3092\u5206\u5b50\u30ec\u30d9\u30eb\u3067\u89e3\u660e\u3057\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u6700\u8fd1\u3001\u3053\u306e\u96fb\u5b50\u4f1d\u9054\u7cfb\u306e\u6700\u7d42\u6bb5\u968e\u3067\u9178\u7d20\u5206\u5b50\u304b\u3089\u6c34\u5206\u5b50\u3092\u751f\u6210\u3055\u305b\u308b<strong>\u30b7\u30c8\u30af\u30ed\u30e0c\u9178\u5316\u9175\u7d20<\/strong>\u3078\u306e\u96fb\u5b50\u4f1d\u9054\u53cd\u5fdc\u306b\u3064\u3044\u3066\u3001<strong>\u591a\u6b21\u5143NMR<\/strong>\u6cd5\u3092\u7528\u3044\u3066\u3001\u305d\u306e\u96fb\u5b50\u53d7\u3051\u53d6\u308a\u306e\u6a5f\u69cb\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u96fb\u5b50\u4f1d\u9054\u53cd\u5fdc\u3067\u306f\u3001\u307e\u305a\u3001\u96fb\u5b50\u904b\u642c\u86cb\u767d\u8cea\u3067\u3042\u308b\u30b7\u30c8\u30af\u30ed\u30e0c\u3068\u3044\u3046\u86cb\u767d\u8cea\u304c\u30b7\u30c8\u30af\u30ed\u30e0c\u9178\u5316\u9175\u7d20\u306b\u7d50\u5408\u3057\u307e\u3059\u304c\u3001<span style=\"text-decoration: underline;\">\u7d50\u5408\u3059\u308b\u3068\u3059\u3050\u306b\u96fb\u5b50\u304c\u53d7\u3051\u6e21\u3055\u308c\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u96fb\u5b50\u304c\u53d7\u3051\u6e21\u3055\u308c\u3084\u3059\u3044\u69cb\u9020\u306b\u5909\u5316\u3057\u3066\u304b\u3089\u96fb\u5b50\u304c\u6e21\u3055\u308c\u3001\u96fb\u5b50\u304c\u30b7\u30c8\u30af\u30ed\u30e0c\u304b\u3089\u30b7\u30c8\u30af\u30ed\u30e0c\u9178\u5316\u9175\u7d20\u306b\u53d7\u3051\u6e21\u3055\u308c\u308b\u3068\u307e\u305f\u69cb\u9020\u5909\u5316\u304c\u8d77\u3053\u308a\u3001\u4eca\u5ea6\u306f\u96fb\u5b50\u304c\u9006\u6d41\u3057\u306a\u3044\u69cb\u9020\u306b\u5909\u5316<\/span>\u3057\u307e\u3059\u3002\u3053\u306e\u7d50\u679c\u304b\u3089\u79c1\u305f\u3061\u306f\u3001\u86cb\u767d\u8cea\u306e\u69cb\u9020\u304c\u5909\u5316\u3059\u308b\u3053\u3068\u3067\u52b9\u7387\u3088\u304f\u96fb\u5b50\u306e\u53d7\u3051\u6e21\u3057\u304c\u3067\u304d\u308b\u6a5f\u69cb\u300c<strong>Gating Mechanism<\/strong>\u300d\u3092\u63d0\u5531\u3057\u3001\u3053\u306e\u3088\u3046\u306a\u6a5f\u69cb\u306fATP\u751f\u7523\u6a5f\u69cb\u306b\u304a\u3044\u3066\u975e\u5e38\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002<\/p><p><span style=\"color: #222222; font-family: arial, sans-serif; font-size: 14px;\">\u00a0<\/span><\/p>","protected":false},"excerpt":{"rendered":"<p>Converting cytochrome c into a DyP-like metalloenzyme, Issei Omura, Koichiro Ishimori and Takeshi Uchida, Dalt [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":30,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/pages\/19"}],"collection":[{"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/comments?post=19"}],"version-history":[{"count":51,"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/pages\/19\/revisions"}],"predecessor-version":[{"id":1793,"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/pages\/19\/revisions\/1793"}],"wp:attachment":[{"href":"https:\/\/wwwchem.sci.hokudai.ac.jp\/~stchem\/wp-json\/wp\/v2\/media?parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}